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验证数据展示

  • WB analysis using 29897-1-AP

    WB analysis using 29897-1-AP

    untreated and IFN alpha treated HeLa cells were subjected to SDS PAGE followed by western blot with 29897-1-AP (IFIT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 29897-1-AP

    IHC staining of human ovary cancer using 29897-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 29897-1-AP (IFIT1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IFIT1 Polyclonal antibody

IFIT1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29897-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

IFIT-1, IFIT 1, IFI-56K, IFI56, IFI 56K

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inIFN alpha treated HeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29897-1-AP targets IFIT1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 IFIT1 fusion protein Ag31980 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 interferon-induced protein with tetratricopeptide repeats 1
别名 IFIT-1, IFIT 1, IFI-56K, IFI56, IFI 56K
计算分子量 478 aa, 55 kDa
观测分子量 55 KDa
GenBank蛋白编号BC007091
基因名称 IFIT1
Gene ID (NCBI) 3434
RRIDAB_3669699
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP09914
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFIT1, also known as glucocorticoid-attenuated response gene 16 protein (GARG-16), is a 463 amino acid protein belonging to the IFIT family. IFIT genes comprise a large family with three (IFIT1, IFIT2, and IFIT3) and four (IFIT1, IFIT2, IFIT3, and IFIT5) members in mice and humans, respectively. IFIT1 specifically bound virus PPP-RNA forms a complex with IFIT2 and IFIT3 to sequester the viral PPP-RNA and prevent virus replication.

实验方案

Product Specific Protocols
WB protocol for IFIT1 antibody 29897-1-APDownload protocol
IHC protocol for IFIT1 antibody 29897-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols