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  • KD/KO Validated

IFIT1 Polyclonal antibody

IFIT1 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, monkey

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 23247-1-AP

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Synonyms

G10P1, GARG 16, IFI 56, IFI 56K, IFI56, IFIT 1, IFIT1, IFNAI1, ISG56, RNM561



经过测试的应用

Positive WB detected inIFN beta treated HeLa cells
Positive IHC detected inhuman kidney tissue, human breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23247-1-AP targets IFIT1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, monkey samples.

Tested Applications IF/ICC, IHC, WB, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse, monkey
Cited Reactivityhuman, mouse
Immunogen IFIT1 fusion protein Ag19691 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interferon-induced protein with tetratricopeptide repeats 1
Synonyms G10P1, GARG 16, IFI 56, IFI 56K, IFI56, IFIT 1, IFIT1, IFNAI1, ISG56, RNM561
Calculated Molecular Weight 478 aa, 55 kDa
Observed Molecular Weight 55 KDa
GenBank Accession NumberBC007091
Gene Symbol IFIT1
Gene ID (NCBI) 3434
RRIDAB_2811269
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDP09914
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFIT1, also known as glucocorticoid-attenuated response gene 16 protein (GARG-16), is a 463 amino acid protein belonging to the IFIT family. IFIT genes comprise a large family with three (IFIT1, IFIT2, and IFIT3) and four (IFIT1, IFIT2, IFIT3, and IFIT5) members in mice and humans, respectively. IFIT1 specifically bound virus PPP-RNA forms a complex with IFIT2 and IFIT3 to sequester the viral PPP-RNA and prevent virus replication.

实验方案

Product Specific Protocols
WB protocol for IFIT1 antibody 23247-1-APDownload protocol
IHC protocol for IFIT1 antibody 23247-1-APDownload protocol
IF protocol for IFIT1 antibody 23247-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Signal Transduct Target Ther

TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion

Authors - Wenwen Zheng
humanIF

Adv Sci (Weinh)

Single-Cell Transcriptomic Analysis of Primary and Metastatic Tumor Ecosystems in Esophageal Squamous Cell Carcinoma

Authors - Yongxu Jia
humanWB

Cancer Lett

Induction of IFIT1/IFIT3 and inhibition of Bcl-2 orchestrate the treatment of myeloma and leukemia via pyroptosis

Authors - Yuanming He
  • KD Validated
humanWB

Int J Mol Sci

Rubella Virus Triggers Type I Interferon Antiviral Response in Cultured Human Neural Cells: Involvement in the Control of Viral Gene Expression and Infectious Progeny Production

Authors - Sayuri Sakuragi
humanWB

J Neuroinflammation

Progression of pathology in PINK1-deficient mouse brain from splicing via ubiquitination, ER stress, and mitophagy changes to neuroinflammation.

Authors - Sylvia Torres-Odio
humanWB,IHC

Cell Oncol (Dordr)

IFIT1 modulates the proliferation, migration and invasion of pancreatic cancer cells via Wnt/β-catenin signaling.

Authors - Tian-Hao Li
  • KD Validated