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验证数据展示

  • IF Staining of HeLa using 29280-1-AP (same clone as 29280-1-PBS)

    IF Staining of HeLa using 29280-1-AP (same clone as 29280-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IFI16 antibody (29280-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29280-1-PBS in a different storage buffer formulation.

  • WB analysis using 29280-1-AP (same clone as 29280-1-PBS)

    WB analysis using 29280-1-AP (same clone as 29280-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29280-1-AP (IFI16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29280-1-PBS in a different storage buffer formulation.

  • IHC staining of human renal cell carcinoma using 29280-1-AP (same clone as 29280-1-PBS)

    IHC staining of human renal cell carcinoma using 29280-1-AP (same clone as 29280-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 29280-1-AP (IFI16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29280-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IFI16 antibody included

IFI16 Polyclonal antibody, PBS Only

IFI16 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29280-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy IFI16 Ready-To-Use IHC Kit. IFI16 primary antibody included.

产品信息

29280-1-PBS targets IFI16 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag30297

Product name: Recombinant human IFI16 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 580-729 aa of BC017059

Sequence: VCRNGFLEVYPFTLVADVNADRNMEIPKGLIRSASVTPKINQLCSQTKGSFVNGVFEVHKKNVRGEFTYYEIQDNTGKMEVVVHGRLTTINCEEGDKLKLTCFELAPKSGNTGELRSVIHSHIKVIKTRKNKKDILNPDSSMETSPDFFF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 interferon, gamma-inducible protein 16
别名
计算分子量 729 aa, 82 kDa
观测分子量85-95 kDa
GenBank蛋白编号BC017059
基因名称 IFI16
Gene ID (NCBI) 3428
RRIDAB_3086109
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16666
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IFI16 is a member of a family of interferon-inducible nuclear proteins involved in transcriptional regulation functions as a transcriptional repressor. IFI16 may be involved in TP53-mediated transcriptional activation by enhancing TP53 sequence-specific DNA binding and modulating TP53 phosphorylation status. It has been reported that IFI16 participates in the regulation of autophagy, TP53-mediated cell death and innate immune response. IFI16 has different isoforms and 67790-1-Ig antibody detects proteins around 85-95 kDa in SDS-PAGE similar to papers. (PMID: 9718316, 14990579, 11146555, 22046441)

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