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验证数据展示

  • WB analysis of mouse brain using 16041-1-AP (same clone as 16041-1-PBS)

    WB analysis of mouse brain using 16041-1-AP (same clone as 16041-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16041-1-AP (IFFO1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16041-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 16041-1-AP (same clone as 16041-1-PBS)

    IHC staining of human brain using 16041-1-AP (same clone as 16041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 16041-1-AP (IFFO1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16041-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 16041-1-AP (same clone as 16041-1-PBS)

    IHC staining of human brain using 16041-1-AP (same clone as 16041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 16041-1-AP (IFFO1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16041-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 16041-1-AP (same clone as 16041-1-PBS)

    IF Staining of HeLa using 16041-1-AP (same clone as 16041-1-PBS)

    Immunofluorescent analysis of Hela cells, using IFFO1 antibody 16041-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 16041-1-PBS in a different storage buffer formulation.

IFFO1 Polyclonal antibody, PBS Only

IFFO1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 16041-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

16041-1-PBS targets IFFO1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8915

Product name: Recombinant human IFFO1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 94-294 aa of BC004384

Sequence: MGGRKRERKAAVEEDTSLSESEGPRQPDGDEEESTALSINEEMQRMLNQLREYDFEDDCDSLTWEETEETLLLWEDFSGYAMAAAEAQGEQQEDSLEKVIKDTESLFKTREKEYQETIDQIELELATAKNDMNRHLHEYMEMCSMKRGLDVQMETCRRLITQSGDRKSPAFTAVPLSDPPPPPSEAEDSDRDVSSDSSMR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 intermediate filament family orphan 1
别名
计算分子量 62 kDa
观测分子量 75-85 kDa
GenBank蛋白编号BC004384
基因名称 IFFO1
Gene ID (NCBI) 25900
RRIDAB_2121676
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ0D2I5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IFFO1, also named as IFFO and Tumor antigen HOM-TES-103, is a member of the intermediate filament family which consists of primordial components of the cytoskeleton and nuclear envelope that are mainly distributed in the nucleus, followed by the cytoskeleton and cytoplasm. IFFO1 in the nucleus was identified to combine with both XRCC4 and the nuclear lamina protein lamin A/C to be involved in nonhomologous DNA end joining (NHEJ). The expression of IFFO1 is associated with tumor progression. IFFO1 could be used as a potential marker to differentiate malignant ascites cell-free tumor DNA (cftDNA) of ovarian cancer from benign controls.

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