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IDS Polyclonal antibody

IDS Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 29375-1-AP

Print datasheet

Synonyms

IDS, iduronate 2 sulfatase, Idursulfase, MPS2, SIDS

验证数据展示

  • WB analysis using 29375-1-AP

    WB analysis using 29375-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29375-1-AP (IDS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29375-1-AP targets IDS in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen IDS fusion protein Ag31132 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name iduronate 2-sulfatase
Synonyms IDS, iduronate 2 sulfatase, Idursulfase, MPS2, SIDS
Calculated Molecular Weight312aa,35 kDa; 550aa,62 kDa
Observed Molecular Weight65-72 kDa
GenBank Accession NumberBC006170
Gene Symbol IDS
Gene ID (NCBI) 3423
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP22304
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Iduronate 2-sulfatase (IDS) is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations causing IDS deficiency in humans result in the lysosomal storage of these glycosaminoglycans and Hunter syndrome, an X chromosome-linked disease. IDS is synthesized as two precursor forms of 76 and 90 kDa that are converted, through a 62 kDa intermediate, to 55 and 45 kDa mature polypeptides due to an internal proteolytic cleavage (PMID: 10838181).

实验方案

Product Specific Protocols
WB protocol for IDS antibody 29375-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols