×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 15576-1-AP (same clone as 15576-1-PBS)

    WB analysis using 15576-1-AP (same clone as 15576-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15576-1-AP (IDH3B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15576-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 15576-1-AP (same clone as 15576-1-PBS)

    IF Staining of HepG2 using 15576-1-AP (same clone as 15576-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using IDH3B antibody (15576-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 15576-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 15576-1-AP (same clone as 15576-1-PBS)

    IHC staining of mouse brain using 15576-1-AP (same clone as 15576-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15576-1-AP (IDH3B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15576-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IDH3B antibody included
  • IHC staining of mouse brain using 15576-1-AP (same clone as 15576-1-PBS)

    IHC staining of mouse brain using 15576-1-AP (same clone as 15576-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15576-1-AP (IDH3B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15576-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IDH3B antibody included

IDH3B Polyclonal antibody, PBS Only

IDH3B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15576-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

H IDHB, NAD(+)-specific ICDH subunit beta, RP46

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy® IDH3B Ready-To-Use IHC Kit. IDH3B primary antibody included.

产品信息

15576-1-PBS targets IDH3B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7965

Product name: Recombinant human IDH3B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-385 aa of BC001960

Sequence: MAALSGVRWLTRALVSAGNPGAWRGLSTSAAAHAASRSQAEDVRVEGSFPVTMLPGDGVGPELMHAVKEVFKAAAVPVEFQEHHLSEVQNMASEEKLEQVLSSMKENKVAIIGKIHTPMEYKGELASYDMRLRRKLDLFANVVHVKSLPGYMTRHNNLDLVIIREQTEGEYSSLEHESARGVIECLKIVTRAKSQRIAKFAFDYATKKGRGKVTAVHKANIMKLGDGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLVGGAGVVPGESYSAEYAVFETGARHPFAQAVGRNIANPTAMLLSASNMLRHLNLEYHSSMIADAVKKVIKVGKVRTRDMGGYSTTTDFIKSVIGHLQTKGS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 isocitrate dehydrogenase 3 (NAD+) beta
别名 H IDHB, NAD(+)-specific ICDH subunit beta, RP46
计算分子量 42 kDa
观测分子量 42 kDa
GenBank蛋白编号BC001960
基因名称 IDH3B
Gene ID (NCBI) 3420
RRIDAB_3741868
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43837
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Isocitrate dehydrogenase 3 (IDH3) is a key enzyme in the mitochondrial tricarboxylic acid (TCA) cycle, which catalyzes the decarboxylation of isocitrate into α-ketoglutarate and concurrently converts NAD+ into NADH. IDH3B(Isocitrate dehydrogenase 3 (NAD+) beta), also named as RP46, is the beta subunit of IDH3 and controls its substrate levels in the TCA cycle, and it is required for sperm mitochondrial metabolism and spermiogenesis (PMID: 18806796, PMID: 35985423). In addition, DH3B expression increased PFKFB3 protein levels and enhanced glucose uptake, and high expression of IDH3B correlated with poor survival in patients with ESCC, suggesting a potential application of IDH3B in prognosis (PMID: 31053633).

||
New chat

Able™

正在加载，请稍候...