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  • Featured Product
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IDH3A Polyclonal antibody

IDH3A Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 15909-1-AP

Print datasheet

Synonyms

IDH3A

验证数据展示

  • WB analysis of human skeletal muscle using 15909-1-AP

    WB analysis of human skeletal muscle using 15909-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15909-1-AP (IDH3A antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 15909-1-AP

    IHC staining of human brain using 15909-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15909-1-AP (IDH3A antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human brain using 15909-1-AP

    IHC staining of human brain using 15909-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15909-1-AP (IDH3A antibody) at dilution of 1:100 (under 10x lens).

  • IP experiment of HepG2 using 15909-1-AP

    IP experiment of HepG2 using 15909-1-AP

    IP result of anti-IDH3A (IP:15909-1-AP, 4ug; Detection:15909-1-AP 1:1000) with HepG2 cells lysate 2400ug.

  • IF Staining of HepG2 using 15909-1-AP

    IF Staining of HepG2 using 15909-1-AP

    Immunofluorescent analysis of HepG2 cells using 15909-1-AP (IDH3A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 15909-1-AP

    WB analysis using 15909-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15909-1-AP (IDH3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 15909-1-AP

    IF Staining of HepG2 using 15909-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using IDH3A antibody (15909-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • FC experiment of HepG2 using 15909-1-AP

    FC experiment of HepG2 using 15909-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human IDH3A (15909-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHepG2 cells, human skeletal muscle tissue, mouse brain tissue, rat brain tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15909-1-AP targets IDH3A in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsIF, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen IDH3A fusion protein Ag8662 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name isocitrate dehydrogenase 3 (NAD+) alpha
Synonyms IDH3A
Calculated Molecular Weight 366 aa, 40 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC021967
Gene Symbol IDH3A
Gene ID (NCBI) 3419
RRIDAB_2123282
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP50213
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IDH3A antibody 15909-1-APDownload protocol
IHC protocol for IDH3A antibody 15909-1-APDownload protocol
IF protocol for IDH3A antibody 15909-1-APDownload protocol
IP protocol for IDH3A antibody 15909-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Nat Neurosci

A glycolytic shift in Schwann cells supports injured axons.

Authors - Elisabetta Babetto
View Article
humanWB

Nat Commun

Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.

Authors - Xiao-Hui Wang
View Article
mouseWB

EMBO J

Asparagine reinforces mTORC1 signaling to boost thermogenesis and glycolysis in adipose tissues.

Authors - Yingjiang Xu
View Article
humanIHC

J Invest Dermatol

Enhanced Glycogen Metabolism Supports the Survival and Proliferation of HPV-Infected Keratinocytes in Condylomata Acuminata.

Authors - Zhichao Gu
View Article
humanWB

Oncotarget

CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.

Authors - Zhichao Gu
View Article
ratWB

Cell Physiol Biochem

Energy Metabolism in the Bone is Associated with Histomorphometric Changes in Rats with Hyperthyroidism.

Authors - Zhuoqing Hu
View Article