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验证数据展示

  • WB analysis using 21803-1-AP

    WB analysis using 21803-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21803-1-AP (ID4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 21803-1-AP

    IHC staining of mouse testis using 21803-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21803-1-AP (ID4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 21803-1-AP

    IHC staining of mouse testis using 21803-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21803-1-AP (ID4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ID4 Polyclonal antibody

ID4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21803-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

bHLHb27, ID4, IDB4, Inhibitor of DNA binding 4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, K-562 cells, MDA-MB-231 cells, mouse testis tissue
Positive IHC detected inmouse testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21803-1-AP targets ID4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag16883

Product name: Recombinant human ID4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-70 aa of BC014941

Sequence: MKAVSPVRPSGRKAPSGCGGGELALRCLAEHGHSLGGSAAAAAAAAAARCKAAEAAADEPALCLQCDMND

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 inhibitor of DNA binding 4, dominant negative helix-loop-helix protein
别名 bHLHb27, ID4, IDB4, Inhibitor of DNA binding 4
计算分子量 161 aa, 17 kDa
观测分子量25-30 kDa
GenBank蛋白编号BC014941
基因名称 ID4
Gene ID (NCBI) 3400
RRIDAB_3085673
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP47928
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ID4 (inhibitor of DNA binding 4) negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. (PMID: 19783986). ID4 levels dictate the stem cell state in mouse spermatogonia (PMID: 28087628). It is involved in the regulation of many cellular processes during both prenatal development and tumorigenesis. ID4 can be detected 25-30 kDa (PMID: 34108007, PMID: 31847356).

实验方案

Product Specific Protocols
WB protocol for ID4 antibody 21803-1-APDownload protocol
IHC protocol for ID4 antibody 21803-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols