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Host
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Species Reactivity
0
Conjugate
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验证数据展示

  • FC experiment of mouse splenocytes using 98058-1-RR (same clone as 98058-1-PBS)

    FC experiment of mouse splenocytes using 98058-1-RR (same clone as 98058-1-PBS)

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse ICOSLG/B7-H2/CD275 Rabbit Recombinant Antibody (98058-1-RR, Clone:240243G5) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD45R (B220). Cells were not fixed. This data was developed using the same antibody clone with 98058-1-PBS in a different storage buffer formulation.

  • FC experiment of mouse splenocytes using 98058-1-RR (same clone as 98058-1-PBS)

    FC experiment of mouse splenocytes using 98058-1-RR (same clone as 98058-1-PBS)

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse ICOSLG/B7-H2/CD275 Rabbit Recombinant Antibody (98058-1-RR, Clone:240243G5) (red) or Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. This data was developed using the same antibody clone with 98058-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98058-1-RR

    Affinity and Kinetic Characterization of 98058-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98058-1-RR against Mouse ICOSLG/B7-H2/CD275 were performed. The affinity constant is below 1 pM.

Anti-Mouse ICOSLG/B7-H2/CD275 Rabbit Recombinant Antibody, PBS Only

ICOSLG/B7-H2/CD275 Uni-rAbTM Recombinant Antibody for FC
Cat No. 98058-1-PBS

产品说明书

CloneNo. 240243G5

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC

别名

CD275, B7-H2, Icosl, ICOS L, GL50

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

98058-1-PBS targets ICOSLG/B7-H2/CD275 in FC applications and shows reactivity with mouse samples.

经测试应用 FC Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 icos ligand
别名 CD275, B7-H2, Icosl, ICOS L, GL50
计算分子量36 kDa
GenBank蛋白编号NM_015790.3
基因名称 CD275
Gene ID (NCBI) 50723
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9JHJ8-1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ICOSLG, also known as B7H2 or GL50, is a type I transmembrane glycoprotein belonging to the B7 ligand family. ICOSLG is extensively expressed on professional antigen-presenting cells including B cells, macrophages, and dendritic cells, as well as non-lymphoid cells including mesenchymal stem cells, endothelial cells, fibroblasts, and tumor cells (PMID: 33756276; 35800767). It is a specific ligand for the T-cell-specific cell surface receptor ICOS and acts as a co-stimulatory molecule (PMID: 11023515; 11007762). The interaction of ICOSLG and ICOS plays important roles in the activation, proliferation, differentiation, and cytokine production of T cells as well as in the antibody secretion from B cells during secondary immune responses (PMID: 21851236).