ICOS Polyclonal antibody
ICOS Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, HL-60 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:200-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
13338-1-AP targets ICOS in WB, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, ELISA |
Tested Reactivity | human |
Immunogen | ICOS fusion protein Ag4155 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | inducible T-cell co-stimulator |
Synonyms | AILIM, CD278, ICOS, inducible T cell co stimulator, Inducible T cell costimulator |
Calculated Molecular Weight | 199 aa, 23 kDa |
Observed Molecular Weight | 28 kDa, 45-50 kDa |
GenBank Accession Number | BC028210 |
Gene Symbol | ICOS |
Gene ID (NCBI) | 29851 |
RRID | AB_2122590 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9Y6W8 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Inducible T cell co-stimulator (ICOS) is related to the CD28 superfamily and is highly expressed on activated T cells as well as regulatory T cells and is crucial for the survival and function of T cells, Th2 cell differentiation, and lung inflammatory responses. Binding ICOS to ICOS-ligand (ICOS-L) activates a cascade of intracellular signaling molecules that prevent apoptosis and lead to the production of cytokines such as IL-4 and IL-13. Catalog#13338-1-AP recognizes two bands of 25-30 kDa and 45-50 kDa, and the additional 45-50 kDa band may be due to surface
disulfide-linked homodimeric glycoprotein.
实验方案
Product Specific Protocols | |
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WB protocol for ICOS antibody 13338-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |