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验证数据展示

  • WB analysis using 11918-1-AP

    WB analysis using 11918-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11918-1-AP (ICAM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

ICAM4 Polyclonal antibody

ICAM4 Polyclonal Antibody for WB, ELISA
Cat No. 11918-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

CD242, ICAM 4, ICAM-4, Landsteiner-Wiener blood group glycoprotein, LW

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected infetal human brain tissue, mouse eye tissue, mouse brain tissue, rat brain tissue
Planning an IHC experiment? We recommend our IHCeasy ICAM4 Ready-To-Use IHC Kit. ICAM4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11918-1-AP targets ICAM4 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2544

Product name: Recombinant human ICAM4 protein

Source: e coli.-derived, T-GST

Tag: GST

Domain: 1-271 aa of BC029364

Sequence: MGSLFPLSLLFFLAAAYPGVGSALGRRTKRAQSPKGSPLAPSGTSVPFWVRMSPEFVAVQPGKSVQLNCSNSCPQPQNSSLRTPLRQGKTLRGPGWVSYQLLDVRAWSSLAHCLVTCAGKTRWATSRITAYKPPHSVILEPPVLKGRKYTLRCHVTQVFPVGYLVVTLRHGSRVIYSESLERFTGLDLANVTLTYEFAAGPRDFWQPVICHARLNLDGLVVRNSSAPITLMLAWSPAPTALASGSIAALVGILLTVGAAYLCKCLAMKSQA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 intercellular adhesion molecule 4 (Landsteiner-Wiener blood group)
别名 CD242, ICAM 4, ICAM-4, Landsteiner-Wiener blood group glycoprotein, LW
计算分子量 271 aa, 29 kDa
观测分子量 42 kDa
GenBank蛋白编号BC029364
基因名称 ICAM4
Gene ID (NCBI) 3386
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14773
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This gene encodes the Landsteiner-Wiener (LW) blood group antigen(s) that belongs to the immunoglobulin (Ig) superfamily, and that shares similarity with the intercellular adhesion molecule (ICAM) protein family. The protein migrate as a 42kd glycoprotein on SDS PAGE. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

实验方案

Product Specific Protocols
WB protocol for ICAM4 antibody 11918-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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