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验证数据展示

  • WB analysis of human peripheral blood platelets using 67628-1-Ig

    WB analysis of human peripheral blood platelets using 67628-1-Ig

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67628-1-Ig (ICAM2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HUVEC using 67628-1-Ig

    IF Staining of HUVEC using 67628-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using ICAM2/CD102 antibody (67628-1-Ig, Clone: 1H3C6 ) at dilution of 1:1991 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

ICAM2/CD102 Monoclonal antibody

ICAM2/CD102 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67628-1-Ig

产品说明书

CloneNo. 1H3C6

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

CD102, ICAM2, ICAM-2, ICAM 2, 1H3C6

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman peripheral blood platelets, Jurkat cells
Positive IF/ICC detected inHUVEC cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:995-1:3982
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67628-1-Ig targets ICAM2/CD102 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag29441

Product name: Recombinant human ICAM2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 65-223 aa of BC003097

Sequence: DKILLDEQAQWKHYLVSNISHDTVLQCHFTCSGKQESMNSNVSVYQPPRQVILTLQPTLVAVGKSFTIECRVPTVEPLDSLTLFLFRGNETLHYETFGKAAPAPQEATATFNSTADREDGHRNFSCLAVLDLMSRGGNIFHKHSAPKMLEIYEPVSDSQ

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 intercellular adhesion molecule 2
别名 CD102, ICAM2, ICAM-2, ICAM 2, 1H3C6
计算分子量 31 kDa
观测分子量 55-60 kDa
GenBank蛋白编号BC003097
基因名称 ICAM2/CD102
Gene ID (NCBI) 3384
RRIDAB_2882829
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP13598
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ICAM2 is a cell adhesion protein having important roles in cell migration, especially during inflammation when leukocytes cross the endothelium. Initially described as a receptor for lymphocyte function-associated antigen-1 (LFA1), ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.

实验方案

Product Specific Protocols
IF protocol for ICAM2/CD102 antibody 67628-1-IgDownload protocol
WB protocol for ICAM2/CD102 antibody 67628-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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