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验证数据展示

  • WB analysis using RMX00016

    WB analysis using RMX00016

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00016 (ICAM-1/CD54 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung squamous cell carcinoma using RMX00016

    IHC staining of human lung squamous cell carcinoma using RMX00016

    Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using RMX00016 (ICAM-1/CD54 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ICAM-1 antibody included
  • IF Staining of Raji using RMX00016

    IF Staining of Raji using RMX00016

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using ICAM-1/CD54 antibody (RMX00016) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Multi-rAb® ICAM-1/CD54 Multi-Recombinant antibody

ICAM-1/CD54 Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. RMX00016

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

CD54, ICAM-1, BB2, ICAM 1, ICAM1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inRaji cells, L02 cells, HUVEC cells
Positive IHC detected inhuman lung squamous cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRaji cells
Planning an IHC experiment? We recommend our IHCeasy ICAM-1 Ready-To-Use IHC Kit. ICAM-1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

RMX00016 targets ICAM-1/CD54 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

Recombinant protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Multi-Recombinant
产品类型 Antibody
全称 intercellular adhesion molecule 1
别名 CD54, ICAM-1, BB2, ICAM 1, ICAM1
计算分子量 90 kDa
观测分子量 85-95 kDa
GenBank蛋白编号BC015969
基因名称 ICAM-1
Gene ID (NCBI) 3383
ENSEMBL Gene IDENSG00000090339
RRIDAB_3750075
偶联类型 Unconjugated
形式Liquid
纯化方式N/A
UNIPROT IDP05362
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).

实验方案

Product Specific Protocols
WB protocol for ICAM-1/CD54 antibody RMX00016Download protocol
IHC protocol for ICAM-1/CD54 antibody RMX00016Download protocol
IF protocol for ICAM-1/CD54 antibody RMX00016Download protocol
Standard Protocols
Click here to view our Standard Protocols
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