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验证数据展示

  • WB analysis using 83696-3-RR

    WB analysis using 83696-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83696-3-RR (ICAM-1/CD54 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat liver using 83696-3-RR

    IHC staining of rat liver using 83696-3-RR

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83696-3-RR (ICAM-1/CD54 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat liver using 83696-3-RR

    IHC staining of rat liver using 83696-3-RR

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83696-3-RR (ICAM-1/CD54 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat liver using 83696-3-RR

    IF Staining of rat liver using 83696-3-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using ICAM-1/CD54 antibody (83696-3-RR, Clone: 240725C2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83696-3-RR

    Affinity and Kinetic Characterization of 83696-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83696-3-RR against Mouse ICAM-1/CD54 were performed. The affinity constant is 1.09 nM.

ICAM-1/CD54 Recombinant antibody

ICAM-1/CD54 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83696-3-RR

产品说明书

CloneNo. 240725C2

宿主/亚型

Rabbit / IgG

种属反应性

rat

应用

WB, IHC, IF-P, ELISA

别名

CD54, ICAM-1, ICAM-12, Icam1, 240725C2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat lung tissue, rat liver tissue, rat heart tissue, rat kidney tissue, rat spleen tissue
Positive IHC detected inrat liver tissue, rat lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat liver tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83696-3-RR targets ICAM-1/CD54 in WB, IHC, IF-P, ELISA applications and shows reactivity with rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 intercellular adhesion molecule 1
别名 CD54, ICAM-1, ICAM-12, Icam1, 240725C2
计算分子量60 kDa
观测分子量85 kDa
GenBank蛋白编号NM_012967.1
基因名称 Icam1
Gene ID (NCBI) 25464
RRIDAB_3671299
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ00238
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ICAM-1 (CD54) is a transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).

实验方案

Product Specific Protocols
WB protocol for ICAM-1/CD54 antibody 83696-3-RRDownload protocol
IHC protocol for ICAM-1/CD54 antibody 83696-3-RRDownload protocol
IF protocol for ICAM-1/CD54 antibody 83696-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols