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验证数据展示

  • WB analysis using 83069-6-RR

    WB analysis using 83069-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83069-6-RR (ICAM-1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 83069-6-RR

    IF Staining of HeLa using 83069-6-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ICAM-1 antibody (83069-6-RR, Clone: 230342A2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83069-6-RR

    Affinity and Kinetic Characterization of 83069-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83069-6-RR against Human ICAM-1 were performed. The affinity constant is 0.271 nM.

ICAM-1/CD54 Recombinant monoclonal antibody

ICAM-1/CD54 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83069-6-RR

产品说明书

CloneNo. 230342A2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

CD54, ICAM-1, ICAM1, ICAM 1, BB2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHUVEC cells, Raji cells, Daudi cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ICAM-1 Ready-To-Use IHC Kit. ICAM-1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83069-6-RR targets ICAM-1/CD54 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

Recombinant protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 intercellular adhesion molecule 1
别名 CD54, ICAM-1, ICAM1, ICAM 1, BB2
计算分子量 90 kDa
观测分子量 85-95 kDa
GenBank蛋白编号BC015969
基因名称 ICAM-1
Gene ID (NCBI) 3383
ENSEMBL Gene IDENSG00000090339
RRIDAB_3670791
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP05362
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).

实验方案

Product Specific Protocols
IF protocol for ICAM-1/CD54 antibody 83069-6-RRDownload protocol
WB protocol for ICAM-1/CD54 antibody 83069-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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