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ICAM-1 Recombinant antibody

ICAM-1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2E23

Cat No : 82827-1-RR

Print datasheet

Synonyms

CD54, ICAM 1, Icam1, Ly 47, MALA 2

验证数据展示

  • WB analysis using 82827-1-RR

    WB analysis using 82827-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82827-1-RR (ICAM-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse lung tissue, mouse spleen tissue, rat lung tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82827-1-RR targets ICAM-1 in WB, ELISA applications and shows reactivity with Mouse, Rat samples.

Tested Applications WB, ELISA
Tested Reactivity Mouse, Rat
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name intercellular adhesion molecule 1
Synonyms CD54, ICAM 1, Icam1, Ly 47, MALA 2
Calculated Molecular Weight59kd
Observed Molecular Weight85-110 kDa
GenBank Accession NumberNM_010493
Gene Symbol Icam1
Gene ID (NCBI) 15894
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13597
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ICAM-1 (CD54) is a transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).

实验方案

Product Specific Protocols
WB protocol for ICAM-1 antibody 82827-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols