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ICA1L Polyclonal antibody

ICA1L Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 18021-1-AP

Print datasheet

Synonyms

ALS2CR14, ALS2CR15, ICA1L

验证数据展示

  • WB analysis using 18021-1-AP

    WB analysis using 18021-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18021-1-AP (ICA1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 18021-1-AP

    IHC staining of human brain using 18021-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 18021-1-AP (ICA1L antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 18021-1-AP

    IHC staining of human brain using 18021-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 18021-1-AP (ICA1L antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inHeLa cells, mouse pancreas tissue, mouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18021-1-AP targets ICA1L in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen ICA1L fusion protein Ag12570 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name islet cell autoantigen 1,69kDa-like
Synonyms ALS2CR14, ALS2CR15, ICA1L
Calculated Molecular Weight 482 aa, 54 kDa
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC063876
Gene Symbol ICA1L
Gene ID (NCBI) 130026
RRIDAB_2121543
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NDH6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ICA1L antibody 18021-1-APDownload protocol
IHC protocol for ICA1L antibody 18021-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols