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验证数据展示

  • WB analysis using 84706-4-RR

    WB analysis using 84706-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84706-4-RR (IBTK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 84706-4-RR

    WB analysis of A549 using 84706-4-RR

    WB result of IBTK antibody (84706-4-RR; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-IBTK transfected A549 cells.

  • IHC staining of mouse lung using 84706-4-RR

    IHC staining of mouse lung using 84706-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 84706-4-RR (IBTK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

IBTK Recombinant monoclonal antibody

IBTK Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 84706-4-RR

产品说明书

CloneNo. 242177D1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

242177D1, BTBD26, BTKI, KIAA1417, RP1 93K22.1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HepG2 cells, Jurkat cells, Ramos cells
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84706-4-RR targets IBTK in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag35421

Product name: Recombinant human IBTK protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 850-1000 aa of BC143745

Sequence: VADQLLITRLKEICEVALTEKLTLKNAAMLLEFAAMYSAKQLKLSCLQFIGLNMAALLEARSLDVLSDGVLKDLSEFYRKMIPAMDRRVITPYQDGPDISYLEVEDGDIFLKEEINMEQNHSETMFKKAKTKAKKKPRKRSDSSGGYNLSD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 inhibitor of Bruton agammaglobulinemia tyrosine kinase
别名 242177D1, BTBD26, BTKI, KIAA1417, RP1 93K22.1
观测分子量160 kDa
GenBank蛋白编号BC143745
基因名称 IBTK
Gene ID (NCBI) 25998
RRIDAB_3743668
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9P2D0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IBTK, also known as BTKI and KIAA1417, which is involved in stress response and tumor growth. It has 3 isoforms and has been found to be hyper-expressed in chronic lymphocytic leukemia and correlated with disease progression (PMID: 29317636).

实验方案

Product Specific Protocols
IHC protocol for IBTK antibody 84706-4-RRDownload protocol
WB protocol for IBTK antibody 84706-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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