IBA1 Monoclonal antibody

IBA1 Monoclonal Antibody for WB, IHC, ELISA, IF

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, ELISA, IF and More (2)

Conjugate

Unconjugated

CloneNo.

1C6A10

Cat No : 66827-1-Ig

Print datasheet

Synonyms

AIF1, G1, allograft inflammatory factor 1, AIF-1, AIF 1



经过测试的应用

Positive IHC detected inhuman brain tissue, human gliomas tissue, human liver cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy IBA1 Ready-To-Use IHC Kit. IBA1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66827-1-Ig targets IBA1 in WB, IHC, ELISA, IF applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA, IF Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen IBA1 fusion protein Ag28236 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name allograft inflammatory factor 1
Synonyms AIF1, G1, allograft inflammatory factor 1, AIF-1, AIF 1
Calculated Molecular Weight 17 kDa
GenBank Accession NumberBC009474
Gene Symbol IBA1
Gene ID (NCBI) 199
ENSEMBL Gene IDENSG00000204472
RRIDAB_2882170
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP55008
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IBA1 is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Expression of IBA1 is up-regulated in activated microglia following facial nerve axotomy, ischemia, and several brain diseases, thereby implicating it in the activated phenotypes of microglia.

实验方案

Product Specific Protocols
IHC protocol for IBA1 antibody 66827-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Nat Commun

Fatal iatrogenic cerebral β-amyloid-related arteritis in a woman treated with lecanemab for Alzheimer's disease

Authors - Elena Solopova
IF

Cell Death Dis

ChemR23 activation attenuates cognitive impairment in chronic cerebral hypoperfusion by inhibiting NLRP3 inflammasome-induced neuronal pyroptosis

Authors - Yaxuan Zhang
IF

Oxid Med Cell Longev

Senkyunolide I Protects against Sepsis-Associated Encephalopathy by Attenuating Sleep Deprivation in a Murine Model of Cecal Ligation and Puncture.

Authors - Jian Xie
IF

J Neuroinflammation

Upregulation of TRPC5 in hippocampal excitatory synapses improves memory impairment associated with neuroinflammation in microglia knockout IL-10 mice.

Authors - Shiji Huo
humanIHC,IF

Brain Pathol

Differential perivascular microglial activation in the deep white matter in vascular dementia developed post-stroke.

Authors - Yoshiki Hase
IF

Mol Neurobiol

Hmox1 is Identified as a Ferroptosis Hub Gene and Associated with the M1 Type Microglia/Macrophage Polarization in Spinal Cord Injury: Bioinformatics and Experimental Validation

Authors - Yong Xuan