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  • IHC staining of human brain using 66827-1-Ig

    IHC staining of human brain using 66827-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IBA1 antibody included
  • IHC staining of human brain using 66827-1-Ig

    IHC staining of human brain using 66827-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IBA1 antibody included
  • IHC staining of human tonsillitis using 66827-1-Ig

    IHC staining of human tonsillitis using 66827-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IBA1 antibody included
  • IHC staining of human tonsillitis using 66827-1-Ig

    IHC staining of human tonsillitis using 66827-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IBA1 antibody included
  • IHC staining of human brain using 66827-1-Ig

    IHC staining of human brain using 66827-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66827-1-Ig (human IBA1 antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human appendicitis using 66827-1-Ig

    IHC staining of human appendicitis using 66827-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66827-1-Ig (human IBA1 antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human liver using 66827-1-Ig

    IHC staining of human liver using 66827-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66827-1-Ig (human IBA1 antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human lung using 66827-1-Ig

    IHC staining of human lung using 66827-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66827-1-Ig (human IBA1 antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human pituitary adenoma using 66827-1-Ig

    IHC staining of human pituitary adenoma using 66827-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 66827-1-Ig (human IBA1 antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human pituitary adenoma using 66827-1-Ig

    IHC staining of human pituitary adenoma using 66827-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 66827-1-Ig (human IBA1 antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human gliomas using 66827-1-Ig

    IHC staining of human gliomas using 66827-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66827-1-Ig (human IBA1 antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human gliomas using 66827-1-Ig

    IHC staining of human gliomas using 66827-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66827-1-Ig (human IBA1 antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human tonsillitis using 66827-1-Ig

    IHC staining of human tonsillitis using 66827-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66827-1-Ig (human IBA1 antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human gliomas using 66827-1-Ig

    IHC staining of human gliomas using 66827-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66827-1-Ig (human IBA1 antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IBA1 antibody included
  • IHC staining of human pituitary adenoma using 66827-1-Ig

    IHC staining of human pituitary adenoma using 66827-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 66827-1-Ig (human IBA1 antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IBA1 antibody included
  • IHC staining of human pituitary adenoma using 66827-1-Ig

    IHC staining of human pituitary adenoma using 66827-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 66827-1-Ig (human IBA1 antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IBA1 antibody included
  • IHC staining of human gliomas using 66827-1-Ig

    IHC staining of human gliomas using 66827-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66827-1-Ig (human IBA1 antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IBA1 antibody included
  • IHC staining of human tonsillitis using 66827-1-Ig

    IHC staining of human tonsillitis using 66827-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66827-1-Ig (human IBA1 antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IBA1 antibody included
  • IHC staining of human appendicitis using 66827-1-Ig

    IHC staining of human appendicitis using 66827-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66827-1-Ig (human IBA1 antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IBA1 antibody included
  • IHC staining of human liver using 66827-1-Ig

    IHC staining of human liver using 66827-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66827-1-Ig (human IBA1 antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IBA1 antibody included
  • IHC staining of human brain using 66827-1-Ig

    IHC staining of human brain using 66827-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66827-1-Ig (human IBA1 antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IBA1 antibody included
  • IHC staining of human lung using 66827-1-Ig

    IHC staining of human lung using 66827-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66827-1-Ig (human IBA1 antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IBA1 antibody included

human IBA1 Monoclonal antibody

human IBA1 Monoclonal Antibody for IHC, ELISA
Cat No. 66827-1-Ig

产品说明书

CloneNo. 1C6A10
引用文献(60)

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

IHC, ELISA and More (2)

别名

AIF1, IBA1, 1C6A10, AIF 1, AIF-1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 647
规格价格库存

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经过测试的应用

Positive IHC detected inhuman brain tissue, human gliomas tissue, human liver cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy IBA1 Ready-To-Use IHC Kit. IBA1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66827-1-Ig targets human IBA1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag28236

Product name: Recombinant human IBA1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-147 aa of BC009474

Sequence: MSQTRDLQGGKAFGLLKAQQEERLDEINKQFLDDPKYSSDEDLPSKLEGFKEKYMEFDLNGNGDIDIMSLKRMLEKLGVPKTHLELKKLIGEVSSGSGETFSYPDFLRMMLGKRSAILKMILMYEEKAREKEKPTGPPAKKAISELP

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 allograft inflammatory factor 1
别名 AIF1, IBA1, 1C6A10, AIF 1, AIF-1
计算分子量 17 kDa
GenBank蛋白编号BC009474
基因名称 IBA1
Gene ID (NCBI) 199
ENSEMBL Gene IDENSG00000204472
RRIDAB_2882170
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP55008
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IBA1 is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Expression of IBA1 is up-regulated in activated microglia following facial nerve axotomy, ischemia, and several brain diseases, thereby implicating it in the activated phenotypes of microglia.

实验方案

Product Specific Protocols
IHC protocol for human IBA1 antibody 66827-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Nat Commun

Fatal iatrogenic cerebral β-amyloid-related arteritis in a woman treated with lecanemab for Alzheimer's disease

Authors - Elena Solopova
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IF

Cell Death Dis

ChemR23 activation attenuates cognitive impairment in chronic cerebral hypoperfusion by inhibiting NLRP3 inflammasome-induced neuronal pyroptosis

Authors - Yaxuan Zhang
View Article
IF

Phytomedicine

Withaferin A protects against epilepsy by promoting LCN2-mediated astrocyte polarization to stopping neuronal ferroptosis

Authors - Zixian Zhou
View Article
IF

Oxid Med Cell Longev

Senkyunolide I Protects against Sepsis-Associated Encephalopathy by Attenuating Sleep Deprivation in a Murine Model of Cecal Ligation and Puncture.

Authors - Jian Xie
View Article
IF

Neural Regen Res

Mitochondrial transplantation confers protection against the effects of ischemic stroke by repressing microglial pyroptosis and promoting neurogenesis

Authors - Li Sun
View Article
IF

J Neuroinflammation

Upregulation of TRPC5 in hippocampal excitatory synapses improves memory impairment associated with neuroinflammation in microglia knockout IL-10 mice.

Authors - Shiji Huo
View Article
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