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验证数据展示

  • IHC staining of rat brain using 30523-1-AP

    IHC staining of rat brain using 30523-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30523-1-AP (IBA1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 30523-1-AP

    IHC staining of rat brain using 30523-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30523-1-AP (IBA1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat brain using 30523-1-AP

    IF Staining of rat brain using 30523-1-AP

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using IBA1 antibody (30523-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

IBA1 Polyclonal antibody

IBA1 Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 30523-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

IHC, IF-P, ELISA

别名

AI607846, AIF 1, Aif1, D17H6S50E, G1, Iba1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inrat brain tissue, mouse brain tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30523-1-AP targets IBA1 in IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Ag30575

Product name: Recombinant mouse AIF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 86-147 aa of NM_019467

Sequence: LKRLIREVSSGSEETFSYSDFLRMMLGKRSAILRMILMYEEKNKEHKRPTGPPAKKAISELP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 allograft inflammatory factor 1
别名 AI607846, AIF 1, Aif1, D17H6S50E, G1, Iba1
计算分子量 17 kDa
GenBank蛋白编号NM_019467
基因名称 IBA1
Gene ID (NCBI) 11629
RRIDAB_3086348
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO70200
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IBA1 is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Expression of IBA1 is up-regulated in activated microglia following facial nerve axotomy, ischemia, and several brain diseases, thereby implicating it in the activated phenotypes of microglia.

实验方案

Product Specific Protocols
IF protocol for IBA1 antibody 30523-1-APDownload protocol
IHC protocol for IBA1 antibody 30523-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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