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验证数据展示

  • IHC staining of rat brain using 10904-1-AP (same clone as 10904-1-PBS)

    IHC staining of rat brain using 10904-1-AP (same clone as 10904-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10904-1-AP (IBA1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10904-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IBA1 antibody included
  • IHC staining of rat brain using 10904-1-AP (same clone as 10904-1-PBS)

    IHC staining of rat brain using 10904-1-AP (same clone as 10904-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10904-1-AP (IBA1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10904-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IBA1 antibody included
  • IHC staining of human tonsillitis using 10904-1-AP (same clone as 10904-1-PBS)

    IHC staining of human tonsillitis using 10904-1-AP (same clone as 10904-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10904-1-AP (IBA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10904-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IBA1 antibody included
  • IHC staining of human tonsillitis using 10904-1-AP (same clone as 10904-1-PBS)

    IHC staining of human tonsillitis using 10904-1-AP (same clone as 10904-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10904-1-AP (IBA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10904-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IBA1 antibody included
  • IF Staining of mouse brain using 10904-1-AP (same clone as 10904-1-PBS)

    IF Staining of mouse brain using 10904-1-AP (same clone as 10904-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using IBA1 antibody (10904-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10904-1-PBS in a different storage buffer formulation.

  • IP experiment of THP-1 using 10904-1-AP (same clone as 10904-1-PBS)

    IP experiment of THP-1 using 10904-1-AP (same clone as 10904-1-PBS)

    IP result of anti-IBA1 (IP:10904-1-AP, 4ug; Detection:10904-1-AP 1:500) with THP-1 cells lysate 2400 ug. This data was developed using the same antibody clone with 10904-1-PBS in a different storage buffer formulation.

  • FC experiment of THP-1 using 10904-1-AP (same clone as 10904-1-PBS)

    FC experiment of THP-1 using 10904-1-AP (same clone as 10904-1-PBS)

    1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human IBA1 (10904-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 10904-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

IBA1 Polyclonal antibody, PBS Only

IBA1 Polyclonal Antibody for IHC, IF-P, FC (Intra), IP, ELISA
Cat No. 10904-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF-P, FC (Intra), IP, ELISA

别名

AIF1, AIF 1, AIF-1, allograft inflammatory factor 1, G1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy IBA1 Ready-To-Use IHC Kit. IBA1 primary antibody included.

产品信息

10904-1-PBS targets IBA1 in IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF-P, FC (Intra), IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1363

Product name: Recombinant human IBA1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-147 aa of BC009474

Sequence: MSQTRDLQGGKAFGLLKAQQEERLDEINKQFLDDPKYSSDEDLPSKLEGFKEKYMEFDLNGNGDIDIMSLKRMLEKLGVPKTHLELKKLIGEVSSGSGETFSYPDFLRMMLGKRSAILKMILMYEEKAREKEKPTGPPAKKAISELP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 allograft inflammatory factor 1
别名 AIF1, AIF 1, AIF-1, allograft inflammatory factor 1, G1
计算分子量 17 kDa
GenBank蛋白编号BC009474
基因名称 IBA1
Gene ID (NCBI) 199
ENSEMBL Gene IDENSG00000204472
RRIDAB_2224377
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP55008
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

What is the molecular weight of IBA1?

The molecular weight of IBA1 is 16.7 kD.


 What is IBA1?

Ionized calcium-binding adaptor molecule 1 (IBA1), also known as Allograft inflammatory factor-1 (AIF-1), is an inflammation-responsive scaffold protein expressed and secreted from macrophages. Microglia response factor (MRF-1) and daintain are also similar, and likely identical, proteins (PMIDs: 29749461, 9630473, 23792284).


 What is the function of IBA1?

IBA1 is necessary for macrophage survival, and it is also a key molecule in proinflammatory activity (PMID: 29749461).


 Where is IBA1 localized?

IBA1 is a cytoplasmic protein, often expressed in immunocytes, macrophages, and microglia. It can be used as a marker for normal (not 'dark') microglia in brain tissue, as IBA1 is expressed by all microglial cell subpopulations (PMIDs: 29749461, 11943136, 26847266, 9630473).


 Is IBA1 upregulated in active immunophages?

Yes; its expression is associated with inflammatory activity (PMID: 29749461).


 How do IBA1-positive microglia differ from microglia that express less or no IBA1?

'Dark' microglia is a recently described phenotype associated with Alzheimer's disease pathology and chronic stress; these dark microglia express decreased IBA1 (often punctiform), and show distinct ultrastructural differences from 'normal' microglia as well as condensed and electron-dense cytoplasm and nucleoplasm. Normal microglia generally display strong, diffuse expression of IBA1. IBA1-positive microglial processes in normal conditions also have a strong tendency to exclusively contact synaptic elements, such as axon terminals, dendritic spines, and synaptic clefts (PMIDs: 29992181, 26847266).


 How is IBA1 related to Alzheimer's disease and pain?

Exacerbated immunoactivity of IBA1, particularly in proximity to amyloid plaques, is prominently featured in AD pathology. IBA1, along with other microglial markers, are also associated with pain, and robust microglial reactions often follow spinal cord injury (PMIDs: 29992181, 23792284).


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