Human PGⅡ ELISA Kit
Sensitivity
0.03 ng/mL
Range
1.56-100 ng/mL
Reactivity
Human
Cat no : KE00090
Synonyms
Gastricsin, Pepsinogen C, PEPSINOGEN, GROUP II, PGC, Progastricsin, progastricsin (pepsinogen C)
Validation Data Gallery
Product Information
KE00090 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The PGⅡ ELISA kit is to be used to detect and quantify protein levels of endogenous PGⅡ. The assay recognizes human PGⅡ. An antibody specific for PGⅡ has been pre-coated onto the microwells. The PGⅡ protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for PGⅡ is added to detect the captured PGⅡprotein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human PGⅡ ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.03 ng/mL |
| Range | 1.56-100 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5225 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 99% | 75%-128% |
| Cell culture supernatants | 92% | 86%-101% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 5.4 | 0.2 | 4.3 |
| 2 | 20 | 25.2 | 0.9 | 3.4 |
| 3 | 20 | 89.9 | 6.0 | 6.7 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 5.6 | 0.2 | 3.1 |
| 2 | 24 | 24.4 | 0.6 | 2.4 |
| 3 | 24 | 80.8 | 4.9 | 6.1 |
Background Information
PepsinogenⅠand Ⅱ, as precursors of pepsin, are produced by gastric mucosa and released into the gastric lumen and peripheral circulation. The advanced inflammation of gastric mucosa and its progression toward atrophic gastritis (AG) in the corpus is associated with a change in serum biomarkers pepsinogenⅠand Ⅱ.(PMID:23496362). PepsinogenⅡ also known as progastricsin or PGC (pepsinogen C), is an aspartic protease expressed primarily in gastric chief cells and is a novel marker of type 2 cells with advantages over many of the current markers used to identify type 2 cells in the developing lung(PMID:14578117). It is involved in proteolysis and peptidolysis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Gastricsin, Pepsinogen C, PEPSINOGEN, GROUP II, PGC, Progastricsin, progastricsin (pepsinogen C) |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
