Human iNOS ELISA Kit
Sensitivity
0.06 ng/mL
Range
0.625-40 ng/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00464 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human iNOS ELISA kit is to be used to detect and quantify protein levels of endogenous human iNOS. The assay recognizes human iNOS. An antibody specific for human iNOS has been pre-coated onto the microwells. The human iNOS protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human iNOS is added to detect the captured human iNOS protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human iNOS ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.06 ng/mL |
| Range | 0.625-40 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4843 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 95% | 82%-108% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 18.67 | 1.07 | 5.71 |
| 2 | 8 | 4.39 | 0.22 | 5.07 |
| 3 | 8 | 2.24 | 0.10 | 4.58 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 18.60 | 1.05 | 5.66 |
| 2 | 16 | 4.29 | 0.23 | 5.42 |
| 3 | 16 | 2.30 | 0.11 | 4.91 |
Background Information
Nitric oxide (NO), which is synthesized by nitric oxide synthase (NOS), is the smallest known bioactive molecule, and can be produced by a variety of cells. NO plays an important role in neurotransmission, vascular function, host defense, and immune regulation. Three NOS isoforms have been identified: neuronal nitric oxide synthase (nNOS), inducible nitric oxide synthase (iNOS), and endothelial nitric oxide synthase (eNOS). iNOS is inducible in many types of cells including epithelial, mesenchymal, and myeloid cells. Induction of iNOS expression varies depending on cell type and species. Aberrant expression of iNOS has been documented in different human tumors including head and neck, breast, colon, stomach, and lung cancer. Increased iNOS expression correlates with tumor grade and angiogenesis in breast cancer patients .
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | INOS,NOS2,EC:1.14.13.39,Hepatocyte NOS,HEP-NOS |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
