Human Tie2 ELISA Kit
Sensitivity
0.3 pg/mL
Range
15.6-1000 pg/mL
Reactivity
Human
Cat no : KE00242
Synonyms
Angiopoietin 1 receptor, CD202B, hTIE2, p140 TEK, TEK, TIE 2, TIE2, VMCM, VMCM1
Validation Data Gallery
Product Information
KE00242 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Tie2 ELISA kit is to be used to detect and quantify protein levels of endogenous Tie2. The assay recognizes human Tie2. An antibody specific for Tie2 has been pre-coated onto the microwells. The Tie2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for Tie2 is added to detect the captured Tie2 protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Tie2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum and Plasma 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.3 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7010 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 106% | 83%-119% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 34.3 | 0.9 | 2.7 |
| 2 | 20 | 133.2 | 5.7 | 4.3 |
| 3 | 20 | 516.2 | 11.3 | 2.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 30.9 | 2.2 | 7.1 |
| 2 | 24 | 128.9 | 3.9 | 3.0 |
| 3 | 24 | 495.6 | 20.2 | 4.1 |
Background Information
Tie2 (also known as TEK) is a tyrosine-protein kinase expressed almost exclusively on endothelial cells. It contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. Tie2 acts as cell-surface receptor for ANGPT1, ANGPT2 and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Mutations in the gene of Tie2 are associated with inherited venous malformations of the skin and mucous membranes. Tie2 can also exist as a soluble form (sTie2) which is released from endothelial cells and present in human blood. The concentration of sTie2 is increased in a range of diseases, including peripheral arterial disease and myocardial infarction.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Angiopoietin 1 receptor, CD202B, hTIE2, p140 TEK, TEK, TIE 2, TIE2, VMCM, VMCM1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
