Human TNFRSF9/CD137 ELISA Kit
Sensitivity
1.0 pg/mL
Range
7.8-500 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00125 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TNFRSF9/CD137 ELISA kit is to be used to detect and quantify protein levels of endogenous TNFRSF9/CD137. The assay recognizes human TNFRSF9/CD137. An antibody specific for TNFRSF9/CD137 has been pre-coated onto the microwells. The TNFRSF9/CD137 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for TNFRSF9/CD137 is added to detect the captured TNFRSF9/CD137 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human TNFRSF9/CD137 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 1.0 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3604 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 97% | 77%-122% |
| Cell culture supernatants | 97% | 89%-106% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 241.1 | 7.1 | 2.9 |
| 2 | 20 | 60.3 | 1.1 | 1.8 |
| 3 | 20 | 13.9 | 0.8 | 5.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 246.6 | 14.5 | 5.9 |
| 2 | 24 | 59.2 | 2.4 | 4.1 |
| 3 | 24 | 14.4 | 0.7 | 4.9 |
Background Information
CD137, also known as TNFRSF9 or 4-1BB, is an inducible T cell surface receptor which belongs to the tumor necrosis factor receptor superfamily. CD137 is a transmembrane protein expressed on the surface of activated T-cells. In addition, activation-dependent expression of CD137 has also been found in B lymphocytes, monocytes, and diverse nonlymphoid cell types. CD137 provides a co-stimulatory signal that enhances the survival, and differentiation of cells, and has a crucial role in the development of CD8 cytotoxic T cells and anti-tumor immunity. Soluble forms of CD137 (sCD137) are generated by differential splicing and are released by activated lymphocytes. Elevated serum sCD137 level has been associated with some diseases, including rheumatoid arthritis, multiple sclerosis, chronic lymphocytic leukemia, and acute coronary syndrome.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | TNFRSF9,4 1BB,4 1BB ligand receptor,4-1BB ligand r |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
