Human TNFRSF6B/DcR3 ELISA Kit
Sensitivity
3.4 pg/mL
Range
39-2500 pg/mL
Reactivity
Human
Cat no : KE00188
Synonyms
DCR3, Decoy receptor 3, Decoy receptor for Fas ligand, DJ583P15.1.1, M68, TNFRSF6B, TR6
Validation Data Gallery
Product Information
KE00188 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TNFRSF6B/DcR3 ELISA kit is to be used to detect and quantify protein levels of endogenous TNFRSF6B/DcR3. The assay recognizes human TNFRSF6B/DcR3. An antibody specific for TNFRSF6B/DcR3 has been pre-coated onto the microwells. The TNFRSF6B/DcR3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human TNFRSF6B/DcR3 is added to detect the captured human TNFRSF6B/DcR3 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human TNFRSF6B/DcR3 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysates 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 3.4 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 8771 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysates | 84% | 75%-96% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,145.1 | 27.5 | 2.4 |
| 2 | 20 | 264.4 | 5.0 | 1.9 |
| 3 | 20 | 62.8 | 2.3 | 3.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,200.1 | 24.8 | 2.1 |
| 2 | 24 | 262.6 | 6.1 | 2.3 |
| 3 | 24 | 55.6 | 2.3 | 4.2 |
Background Information
Tumor necrosis factor receptor superfamily member 6B (TNFRSF6B), also identified as DcR3 or M68, is a secreted member of the Tumor Necrosis Factor (TNF) receptor superfamily, neutralizes three different TNF ligands: TNFS14/LIGHT, TNFSF15/TL1A and TNFSF6/FasL. TNFRSF6B binds to FasL and inhibits FasL-induced apoptosis. It can also bind to LIGHT and suppresse LIGHT-mediated apoptosis. Interaction of TL1A with DR3 promotes T cell expansion during an immune response. Expression of DcR3 is significantly elevated in patients with a range of cancers and autoimmune diseases.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | DCR3, Decoy receptor 3, Decoy receptor for Fas ligand, DJ583P15.1.1, M68, TNFRSF6B, TR6 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
