Human GITR/TNFRSF18 ELISA Kit
Sensitivity
11.5 pg/mL
Range
62.5-4000 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00220 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TNFRSF18 ELISA kit is to be used to detect and quantify protein levels of endogenous TNFRSF18. The assay recognizes human TNFRSF18. An antibody specific for TNFRSF18 has been pre-coated onto the microwells. The TNFRSF18 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for TNFRSF18 is added to detect the captured TNFRSF18 protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human GITR/TNFRSF18 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 11.5 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 8784 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 83% | 74%-97% |
| Human plasma | 94% | 75%-112% |
| Cell culture supernatants | 110% | 95%-124% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 165.7 | 2.2 | 1.3 |
| 2 | 20 | 663.9 | 13.3 | 2.0 |
| 3 | 20 | 2,812.0 | 99.6 | 3.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 169.7 | 7.2 | 4.3 |
| 2 | 24 | 694.1 | 19.8 | 2.8 |
| 3 | 24 | 2,957.6 | 107.8 | 3.6 |
Background Information
Glucocorticoid-induced TNFR-related protein (GITR), also known as CD357 or TNFRSF18, is a member of the tumor necrosis factor receptor (TNF-R) superfamily. GITR is expressed constitutively at high levels in T regulatory cells (Treg cells) and plays a key role in dominant immunological self-tolerance maintained by CD25+CD4+ regulatory T cells. It is expressed at low levels on resting responder T cells. The expression of GITR on T cells can be upregulated upon activation. GITR is activated by GITR ligand (GITRL) which is mainly expressed on APC. GITR-GITRL interactions could co-stimulate both responder T-cell functions and the suppressive functions of Treg cells.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AITR, CD357, GITR, GITR D, TNFRSF18 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
