Human TK1 ELISA Kit
Sensitivity
24.7 pg/mL
Range
78.1-5000 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00285 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human TK1 ELISA kit is to be used to detect and quantify protein levels of endogenous human TK1. The assay recognizes human TK1. An antibody specific for human TK1 has been pre-coated onto the microwells. The human TK1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human TK1 is added to detect the captured human TK1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human TK1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 24.7 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7083 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| serum | 91% | 82%-101% |
| Cell culture supernatant | 109% | 91%-124% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 2,484.3 | 187.0 | 7.5 |
| 2 | 20 | 530.4 | 21.7 | 4.1 |
| 3 | 20 | 141.5 | 8.2 | 5.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 2,464.1 | 193.4 | 7.9 |
| 2 | 24 | 538.7 | 42.8 | 8.0 |
| 3 | 24 | 139.4 | 9.5 | 6.8 |
Background Information
Thymidine kinase 1 (TK1) is a cytosolic enzyme involved in pyrimidine metabolism that catalyzes the addition of a gamma-phosphate group to thymidine and in regenerating thymidine for DNA synthesis and DNA damage. Among the four deoxyribonucleoside-specific kinases in mammalian cells, TK1 is the only one with the most restricted substrates specificity. TK1 has been applied as an important biomarker for the diagnosis of various cancers, including breast cancer, esophageal cancer, and lung cancer. TK1 upregulation was indicated as an early event in a study of breast cancer and further studies demonstrated a positive correlation between TK1 and cancer stage.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | thymidine kinase 1, soluble, Thymidine kinase, cytosolic, TK1, TK2 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
