Human TIMP1 ELISA Kit
Sensitivity
3.9 pg/mL
Range
31.25-2000 pg/mL
Reactivity
Human
Cat no : KE00833
Synonyms
TIMP-1, CLGI, Collagenase inhibitor, EPA, Erythroid-potentiating activity
Validation Data Gallery
Product Information
KE00833 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human TIMP1 ELISA kit is to be used to detect and quantify protein levels of endogenous human TIMP1. The assay recognizes human TIMP1. An antibody specific for human TIMP1 has been pre-coated onto the microwells. The human TIMP1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human TIMP1 is added to detect the captured human TIMP1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human TIMP1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Saliva |
| Assay type | Sandwich |
| Sensitivity | 3.9 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7076 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 103% | 97%-109% |
| Cell culture supernatant | 110% | 105%-114% |
| Saliva | 108% | 101%-114% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,055.6 | 32.5 | 3.1 |
| 2 | 8 | 256.9 | 9.8 | 3.8 |
| 3 | 8 | 129.8 | 6.1 | 4.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,016.6 | 53.0 | 5.2 |
| 2 | 16 | 255.2 | 10.0 | 3.9 |
| 3 | 16 | 130.4 | 4.8 | 3.7 |
Background Information
Metalloproteinase inhibitor 1 (TIMP1) is also named as Erythroid-potentiating activity (EPA), Fibroblast collagenase inhibitor (Collagenase inhibitor) and CLGI. TIMP1 is one of the tissue inhibitor members of the metalloproteinase family. TIMP1 regulates the balance of matrix remodeling during the degradation of extracellular matrix by inhibiting the proteolytic activity of matrix metalloproteinases (MMPs). TIMP1 promotes the occurrence and development of GC, which is consistent with previous reports about TIMP1 as an oncogene in other cancers, including breast cancer, colon cancer, glioblastoma, and non-small cell lung cancer. TIMP1 is reportedly as a tumor-secreted protein that could be detected in the serum. In a diabetic environment, TIMP1 initially binds to TFRC, leading to increased TFRC expression, thereby heightening osteoblast sensitivity to ferroptosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | TIMP-1, CLGI, Collagenase inhibitor, EPA, Erythroid-potentiating activity |