Human TIMP1 ELISA Kit
Cat no : KE00833
Synonyms
TIMP-1, CLGI, Collagenase inhibitor, EPA, Erythroid-potentiating activity
Validation Data Gallery
Product Information
KE00833 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human TIMP1 ELISA kit is to be used to detect and quantify protein levels of endogenous human TIMP1. The assay recognizes human TIMP1. An antibody specific for human TIMP1 has been pre-coated onto the microwells. The human TIMP1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human TIMP1 is added to detect the captured human TIMP1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human TIMP1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Saliva 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 3.9 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7076 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 103% | 97%-109% |
| Cell culture supernatant | 110% | 105%-114% |
| Saliva | 108% | 101%-114% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,055.6 | 32.5 | 3.1 |
| 2 | 8 | 256.9 | 9.8 | 3.8 |
| 3 | 8 | 129.8 | 6.1 | 4.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,016.6 | 53.0 | 5.2 |
| 2 | 16 | 255.2 | 10.0 | 3.9 |
| 3 | 16 | 130.4 | 4.8 | 3.7 |
Background Information
Metalloproteinase inhibitor 1 (TIMP1) is also named as Erythroid-potentiating activity (EPA), Fibroblast collagenase inhibitor (Collagenase inhibitor) and CLGI. TIMP1 is one of the tissue inhibitor members of the metalloproteinase family. TIMP1 regulates the balance of matrix remodeling during the degradation of extracellular matrix by inhibiting the proteolytic activity of matrix metalloproteinases (MMPs). TIMP1 promotes the occurrence and development of GC, which is consistent with previous reports about TIMP1 as an oncogene in other cancers, including breast cancer, colon cancer, glioblastoma, and non-small cell lung cancer. TIMP1 is reportedly as a tumor-secreted protein that could be detected in the serum. In a diabetic environment, TIMP1 initially binds to TFRC, leading to increased TFRC expression, thereby heightening osteoblast sensitivity to ferroptosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | TIMP-1, CLGI, Collagenase inhibitor, EPA, Erythroid-potentiating activity |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Cells Integrin-Mediated TIMP1 Signaling Reprograms Liver Macrophages and Accelerates Colorectal Cancer Metastasis. | ||
Commun Biol Succinate-driven PKM2 succinylation and dimerization accelerates age-associated cardiac fibrosis. | ||
Mediators Inflamm Benzydamine Attenuates Matrix Metalloproteinase-9 Expression Through Inhibition of ERK MAPK in Activated Human Monocytic Cells Under Hyperglycemic Condition. |
