Human TFPI ELISA Kit
Sensitivity
7.2 pg/mL
Range
15.6-1000 pg/mL , 62.5-2000 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00202 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TFPI ELISA kit is to be used to detect and quantify protein levels of endogenous TFPI. The assay recognizes human TFPI. An antibody specific for TFPI has been pre-coated onto the microwells. The TFPI protein in samples is captured by the coated antibody after incubation.Following extensive washing, another antibody of biotinylated specific for TFPI is added to detect the captured TFPI protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human TFPI ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 7.2 pg/mL |
| Range | 15.6-1000 pg/mL , 62.5-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7035 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 75%-122% | 107% |
| Cell culture supernatants | 72%-120% | 89% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 23.3 | 2.2 | 9.4 |
| 2 | 20 | 75.7 | 5.4 | 7.1 |
| 3 | 20 | 312.9 | 15.6 | 5.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 22.9 | 1.5 | 6.6 |
| 2 | 24 | 66.8 | 5.6 | 8.5 |
| 3 | 24 | 314.2 | 18.1 | 5.8 |
Background Information
Tissue factor pathway inhibitor (TFPI) is a critical anticoagulant protein present in endothelium and platelets. TFPI is produced as two major isoforms in humans, TFPIa and TFPIb that result from alternative splicing. The TFPIb isoform localizes to the endothelium surface where it is a potent inhibitor of tissue factor-factor VIIa complexes that initiate blood coagulation. The TFPIa isoform is present in platelets. TFPI is a Kunitz-type serine protease inhibitor that exerts anticoagulant activity by blocking early procoagulant stimulia. TFPI can enhance anti-thrombotic treatment in sepsis, inflammatory diseases, and cardiovascular diseases. In plasma, 80% of TFPI is carboxy-terminal truncated and circulates bound primarily to low-density lipoproteins (LDL), with the levels of the latter having an impact on plasma TFPI levels. The other 20% is unbound free-form TFPI characterised by the K3 domain.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EPI, Extrinsic pathway inhibitor, LACI, TFI, TFPI, TFPI1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
