Human TDP-43 ELISA Kit
Cat no : KE00009
Synonyms
ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43
Validation Data Gallery
Product Information
KE00009 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TDP-43 ELISA kit is to be used to detect and quantify protein levels of endogenous TDP-43. The assay recognizes human TDP-43. An antibody specific for TDP-43 has been pre-coated onto the microwells. The TDP-43 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for TDP-43 is added to detect the captured TDP-43 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human TDP-43 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Human cerebrospinal fluid, Cell lysates 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.26 ng/mL |
| Range | 6.25-400 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 23435 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| human cerebrospinal fluid | 109% | 95%-128% |
| cell lysates | 95% | 79%-121% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 37.2 | 1.6 | 4.2 |
| 2 | 20 | 73.0 | 2.0 | 2.8 |
| 3 | 20 | 144.9 | 6.5 | 4.5 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 37.5 | 3.1 | 8.4 |
| 2 | 24 | 72.9 | 3.2 | 4.4 |
| 3 | 24 | 153.8 | 7.2 | 4.6 |
Background Information
TARDBP was firstly found as a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. It was also reported to regulate alternate splicing of the CFTR gene. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP43, is the major disease protein in ubiquitin-positive, tau-, and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in Amyotrophic lateral sclerosis (ALS). KE00009 is used to quantify TDP-43 level in vivo and it is suitable for sample of CSF and cell lysates. KE00005 is used to quantify TDP-43 level in vivo and it is suitable for sample of serum or plasma.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Analyst Diagnosis of idiopathic amyotrophic lateral sclerosis using Fourier-transform infrared spectroscopic analysis of patient-derived skin. | ||
Adv Sci (Weinh) Quantitative Profiling of Nanoscopic Protein Aggregates Reveals Specific Fingerprint of TDP-43-Positive Assemblies in Motor Neuron Disease. |
