Human SOX4 ELISA Kit
Sensitivity
5.7 pg/mL
Range
62.5-4000 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00509 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human SOX4 ELISA kit is to be used to detect and quantify protein levels of endogenous human SOX4. The assay recognizes human SOX4. An antibody specific for human SOX4 has been pre-coated onto the microwells. The human SOX4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human SOX4 is added to detect the captured human SOX4 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human SOX4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 5.7 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6659 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysates | 109% | 109%-110% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1795.1 | 71.7 | 4.0 |
| 2 | 8 | 387.3 | 19.4 | 5.0 |
| 3 | 8 | 100.1 | 4.8 | 4.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,791.7 | 72.6 | 4.0 |
| 2 | 16 | 383.5 | 17.6 | 4.6 |
| 3 | 16 | 100.5 | 6.2 | 6.2 |
Background Information
SOX4 is a transcription factor belonging to the SOX family, which is crucial for embryonic development and cell fate determination. It is located on chromosome 6p22.3 in humans and encodes a protein of 474 amino acids with three distinguishable domains: a high mobility group (HMG) box, a glycine-rich region, and a serine-rich region. This protein regulates gene expression by binding to DNA and interacting with other proteins, thereby influencing cell proliferation, differentiation, and apoptosis. SOX4 has been implicated in various diseases, including cancer and atherosclerosis, due to its role in promoting cell proliferation and survival. For instance, SOX4 is highly expressed in several cancers, such as non-small cell lung cancer (NSCLC), and is associated with poor prognosis. It can induce epithelial-mesenchymal transition (EMT), enhancing cancer cell invasion and metastasis. Additionally, SOX4 contributes to atherosclerosis by regulating endothelial cell function. Its diverse functions and involvement in multiple signaling pathways make SOX4 a significant focus in both basic research and clinical studies.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CSS10, EVI16, Transcription factor SOX-4 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
