Recombinant Human SMN protein (rFc Tag)
种属
Human
纯度
>90 %, SDS-PAGE
标签
rFc Tag
生物活性
未测试
验证数据展示
产品信息
| 纯度 | >90 %, SDS-PAGE |
| 内毒素 | <0.1 EU/μg protein, LAL method |
| 生物活性 |
Not tested |
| 来源 | HEK293-derived Human SMN protein Gln90-Asn151 (Accession# Q16637) with a rabbit IgG Fc tag at the C-terminus |
| 基因ID | 6607 |
| 蛋白编号 | Q16637 |
| 预测分子量 | 33.2 kDa |
| SDS-PAGE | 34-44 kDa, reducing (R) conditions |
| 组分 | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| 复溶 | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| 储存条件 |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
背景信息
Survival Motor Neuron (SMN) protein required for efficient assembly of small nuclear ribonucleoprotein (snRNP) complexes is encoded by nearly identical telomeric and centromeric forms of SMN gene respectively. Both the SMN1 and SMN2 genes express SMN protein, however, the amount of functional full-length protein produced by SMN2 is much less than that produced by SMN1 due to the alternative splicing. The absence of SMN1 can be partially compensated for by SMN2 and the SMN2 expression level is associated with SMA severity.
参考文献:
1. Lefebvre S, et al. (1995). Cell. 80(1):155-65. 2. Gavrilov DK, et al. (1998). Nat Genet. 20(3):230-1. 3. Monani UR, et al. (2000). Hum Mol Genet. 9(3):333-9. 4. Burghes AH, et al. (2009). Nat Rev Neurosci.10(8):597-609.
