Human SMARCA2 ELISA Kit
Sensitivity
0.6 pg/mL
Range
31.25-2000 pg/mL
Reactivity
Human
Cat no : KE00806
Synonyms
ATP-dependent helicase SMARCA2, BAF190, BAF190B, BRG1-associated factor 190B, BRM
Validation Data Gallery
Product Information
KE00806 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human SMARCA2 ELISA kit is to be used to detect and quantify protein levels of endogenous human SMARCA2. The assay recognizes human SMARCA2. An antibody specific for human SMARCA2 has been pre-coated onto the microwells. The human SMARCA2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human SMARCA2 is added to detect the captured human SMARCA2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human SMARCA2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.6 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6595 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 99% | 88%-109% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 865.9 | 33.3 | 3.8 |
| 2 | 8 | 240.8 | 4.4 | 1.8 |
| 3 | 8 | 121.7 | 6.8 | 5.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 835.5 | 69.4 | 8.3 |
| 2 | 16 | 237.1 | 6.5 | 2.7 |
| 3 | 16 | 116.8 | 7.1 | 6.0 |
Background Information
SMARCA2 (also known as BRM) is an important chromatin remodeling protein and a core subunit of the SWI/SNF complex. It alters chromatin structure through its ATPase activity to regulate gene expression and is involved in various biological processes, including cell differentiation, proliferation, and DNA damage repair. SMARCA2 and SMARCA4 (BRG1) are mutually exclusive in different SWI/SNF complexes and work synergistically in the regulation of gene expression. Dysfunction of SMARCA2 is associated with multiple diseases, including various cancers (such as pancreatic cancer, lung cancer, and ovarian cancer) and genetic disorders (such as Nicolaides-Baraitser syndrome).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ATP-dependent helicase SMARCA2, BAF190, BAF190B, BRG1-associated factor 190B, BRM |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
