Human SFRP1 ELISA Kit
Sensitivity
0.009 ng/mL
Range
0.156-10 ng/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00266 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The SFRP1 ELISA kit is to be used to detect and quantify protein levels of endogenous SFRP1. The assay recognizes human SFRP1. An antibody specific for human SFRP1 has been pre-coated onto the microwells. The human SFRP1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human SFRP1 is added to detect the captured human SFRP1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human SFRP1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Human serum, plasma 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.009 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6422 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 107% | 94%-123% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 6.8 | 0.3 | 5.2 |
| 2 | 20 | 1.5 | 0.1 | 5.8 |
| 3 | 20 | 0.7 | 0.03 | 4.9 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 7.6 | 0.3 | 4.5 |
| 2 | 24 | 1.7 | 0.2 | 9.2 |
| 3 | 24 | 0.7 | 0.1 | 9.5 |
Background Information
Secreted Frizzled Related Protein 1 (SFRP1) belongs to the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRP1 has been classified as a tumor suppressor gene due to the loss of expression in various human cancers, which is mainly attributed by epigenetic inactivation via DNA methylation or transcriptional silencing by microRNAs. SFRP1 is a biomarker for aggressive subgroups of human gastric cancer and loss of SFRP1 expression is associated with poor prognosis in hepatocellular carcinoma. Patients with high SFRP1 expression in tumor tissue had a significantly better overall survival rate, and SFRP1 can be used as a prognostic biomarker for patients with poor survival.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | FRP, FRP 1, FRP1, FrzA, SARP 2, SARP2, sFRP 1, SFRP1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
