Human RBP4 ELISA Kit
Sensitivity
0.083 ng/mL
Range
1.56-100 ng/mL
Reactivity
Human
Cat no : KE00270
Synonyms
Plasma retinol binding protein, PRBP, RBP, RBP4, Retinol binding protein 4
Validation Data Gallery
Product Information
KE00270 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The RBP4 ELISA kit is to be used to detect and quantify protein levels of endogenous RBP4. The assay recognizes human RBP4. An antibody specific for RBP4 has been pre-coated onto the microwells. The RBP4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for RBP4 is added to detect the captured RBP4 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human RBP4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Saliva, Urine 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.083 ng/mL |
| Range | 1.56-100 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5950 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 88 | 74-99 |
| Cell culture supernatants | 82 | 72-92 |
| Urine | 89 | 73-104 |
| Saliva | 101 | 86-119 |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 12.3 | 0.65 | 5.3 |
| 2 | 20 | 18.4 | 0.75 | 4.0 |
| 3 | 20 | 40.8 | 0.92 | 2.3 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 12.6 | 0.25 | 2.0 |
| 2 | 24 | 18.0 | 0.60 | 3.3 |
| 3 | 24 | 37.9 | 1.68 | 4.4 |
Background Information
RBP4 (retinol-binding protein 4) belongs to the lipocalin family and is the specific carrier for retinol (Vitamin A) in the blood. It is mainly synthesized in the liver and circulates in the bloodstream bound to retinol in a complex with transthyretin. RBP4 acts as a drug target for ophthalmology research and reduction in serum RBP4 level inhibits lipofuscin without inhibiting the visual cycle. Urinary RBP4 could act as a marker for glomerular disease. And RBP4 also was identified as an adipokine that elevated in some insulin-resistant states. Measurement of serum RBP4 could be used to assess the risk of insulin resistance, type 2 diabetes, obesity, and cardiovascular disease.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Plasma retinol binding protein, PRBP, RBP, RBP4, Retinol binding protein 4 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
