Recombinant Human Plasma kallikrein/KLKB1 protein (His Tag)
种属
Human
纯度
>90 %, SDS-PAGE
标签
His Tag
生物活性
未测试
验证数据展示
产品信息
| 纯度 | >90 %, SDS-PAGE |
| 内毒素 | <1.0 EU/μg protein, LAL method |
| 生物活性 |
Not tested |
| 来源 | HEK293-derived Human Plasma kallikrein/KLKB1 protein Gly20-Ala638 (Accession# P03952, Ser143Asn) with a His tag at the C-terminus. |
| 基因ID | 3818 |
| 蛋白编号 | P03952 |
| 预测分子量 | 70.3 kDa |
| SDS-PAGE | |
| 组分 | Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| 复溶 | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| 储存条件 |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
背景信息
Human Plasma Kallikrein, a serine protease also named as KLKB1, KLK3, PPK or Kininogenin, is synthesized in the liver and circulates in the plasma by binding to high molecular weight (HMW) kininogen or as a free zymogen. It cleaves HMW kininogen, its major physiological substrate, to release the potent vasodilator peptide bradykinin. It is also able to cleave a number of inactive precursor proteins to generate active products, such as plasminogen and prourokinase. Thus, it plays an important role in blood pressure regulation, fibrinolysis, and neutrophil activation.
参考文献:
1. Sainz, Irma M et al. Thrombosis and haemostasis vol. 98,1 (2007): 77-83. 2. Neth, P et al. Thrombosis and haemostasis vol. 85,6 (2001): 1043-7. 3. Fink, Edwin et al. Biological chemistry vol. 388,9 (2007): 957-63. 4. Peek, Mark et al. The Journal of biological chemistry vol. 277,49 (2002): 47804-9.