Human/Mouse Perilipin 1 ELISA Kit
Sensitivity
0.2 pg/mL
Range
39-2500 pg/mL
Reactivity
Human/Mouse
Validation Data Gallery
Product Information
KE00417 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human/mouse LEPR ELISA kit is to be used to detect and quantify protein levels of endogenous human/mouse LEPR. The assay recognizes human/mouse LEPR. An antibody specific for human/mouse LEPR has been pre-coated onto the microwells. The human/mouse LEPR protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human/mouse LEPR is added to detect the captured human/mouse LEPR protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Mouse Perilipin 1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.2 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Human/Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5346 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 78% | 77%-79% |
| Tissue lysate | 92% | 89%-95% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,122.3 | 24.4 | 2.2 |
| 2 | 20 | 267.2 | 7.4 | 2.8 |
| 3 | 20 | 63.6 | 3.3 | 5.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,188.8 | 42.8 | 3.6 |
| 2 | 24 | 284.8 | 10.0 | 3.5 |
| 3 | 24 | 71.9 | 9.1 | 12.6 |
Background Information
PLIN1, also known as Perilipin 1, PLIN, PERI, belongs to the perilipin family. PLIN1 is a hormonally-regulated phosphoprotein that encircles the lipid storage droplet in adipocytes and is a modulator of adipocyte lipid metabolism. PLIN1 is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in the inhibition of lipolysis. PLIN1 is detected in adipocytes of white adipose tissue, visceral adipose tissue and mammary gland. The deficiency of PLIN1 causes lipodystrophic syndromes, which disables the optimal accumulation of triglycerides in adipocytes that derives in an abnormal deposition of lipids in tissues such as skeletal muscle and liver. The storage of lipids in the liver leads to insulin resistance and hypertriglyceridemia.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | PLIN, PERI, PLIN1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
