Speedy™ Human Pentraxin 3/TSG-14 One-Step ELISA Kit
Sensitivity
0.1 pg/mL
Range
23.4-1500 pg/mL
Reactivity
Human
Cat no : SE50022
Synonyms
Pentraxin 3, PTX3, Pentaxin related protein PTX3, Pentaxin-related protein PTX3, Pentraxin related protein PTX3
Validation Data Gallery
Product Information
| Product name | Speedy™ Human Pentraxin 3/TSG-14 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum,Plasma,Cell culture supernatants,Saliva,Cell lysates 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.1 pg/mL |
| Range | 23.4-1500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5806 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatant | 87% | 84%-91% |
| Saliva | 100% | 85%-129% |
| Cell lysate | 79% | 73%-90% |
| Human plasma | 95% | 78%-107% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 793.1 | 49.4 | 6.2 |
| 2 | 8 | 176.5 | 5.6 | 3.2 |
| 3 | 8 | 88.5 | 6.3 | 7.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 782.1 | 42.5 | 5.4 |
| 2 | 16 | 177.2 | 8.0 | 4.5 |
| 3 | 16 | 86.1 | 5.2 | 6.0 |
Background Information
Pentraxin 3 (PTX3), also known as TNF-inducible gene 14 protein (TSG-14), is a member of the pentraxin superfamily which consists of evolutionarily conserved proteins characterized by a structural motif, the pentraxin domain. Pentraxin 3 can be produced by a variety of cell types including endothelial cells, smooth muscle cells, adipocytes, fibroblasts, mononuclear phagocytes, and dendritic cells, and its expression is induced by primary inflammatory signals, such as IL-1, TNF, and microbial moieties. Pentraxin 3 is an acute phase glycoprotein that plays a role in the regulation of innate resistance to pathogens, inflammation, tissue remodeling and repair, female fertility and cancer. Pentraxin 3 is a biomarker for cardiovascular disease. Increased plasma Pentraxin 3 levels have been found in patients with cardiovascular disorders.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Pentraxin 3, PTX3, Pentaxin related protein PTX3, Pentaxin-related protein PTX3, Pentraxin related protein PTX3 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
2、我没有恒温振荡器，这会对实验时间和结果产生影响吗？
如果您没有恒温振荡器，完成这个实验大约需要 2.5 小时。在加入抗体混合物后，我们建议您将板条37℃下静置孵育2小时。在加入 TMB 后，您只需让板条37℃下静置孵育15分钟即可。我们的研发人员通过对比实验已经证明，尽管信号会略有降低，但您仍然可以获得有效的实验数据。
