Human Pentraxin 3/TSG-14 ELISA Kit
Cat no : KE00205
Synonyms
Pentraxin 3, PTX3, Pentaxin related protein PTX3, Pentaxin-related protein PTX3, Pentraxin related protein PTX3
Validation Data Gallery
Product Information
KE00205 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Pentraxin 3/TSG-14 ELISA kit is to be used to detect and quantify protein levels of endogenous Pentraxin 3/TSG-14. The assay recognizes human Pentraxin 3/TSG-14. An antibody specific for Pentraxin 3/TSG-14 has been pre-coated onto the microwells. The Pentraxin 3/TSG-14 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human Pentraxin 3/TSG-14 is added to detect the captured human Pentraxin 3/TSG-14 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Pentraxin 3/TSG-14 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Saliva 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.003 ng/mL |
| Range | 0.027-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5806 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 87% | 75%-104% |
| Human plasma | 86% | 77%-101% |
| saliva | 89% | 76%-113% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 5.63 | 0.24 | 4.2 |
| 2 | 20 | 0.93 | 0.03 | 3.2 |
| 3 | 20 | 0.11 | 0.01 | 4.8 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 5.59 | 0.36 | 6.5 |
| 2 | 24 | 0.94 | 0.04 | 3.8 |
| 3 | 24 | 0.15 | 0.01 | 6.7 |
Background Information
Pentraxin 3 (PTX3), also known as TNF-inducible gene 14 protein (TSG-14), is a member of the pentraxin superfamily which consists of evolutionarily conserved proteins characterized by a structural motif, the pentraxin domain. Pentraxin 3 can be produced by a variety of cell types including endothelial cells, smooth muscle cells, adipocytes, fibroblasts, mononuclear phagocytes, and dendritic cells, and its expression is induced by primary inflammatory signals, such as IL-1, TNF, and microbial moieties. Pentraxin 3 is an acute phase glycoprotein that plays a role in the regulation of innate resistance to pathogens, inflammation, tissue remodeling and repair, female fertility and cancer. Pentraxin 3 is a biomarker for cardiovascular disease. Increased plasma Pentraxin 3 levels have been found in patients with cardiovascular disorders.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Pentraxin 3, PTX3, Pentaxin related protein PTX3, Pentaxin-related protein PTX3, Pentraxin related protein PTX3 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Antibiotics (Basel) Evaluation of Five Host Inflammatory Biomarkers in Early Diagnosis of Ventilator-Associated Pneumonia in Critically Ill Children: A Prospective Single Center Cohort Study | ||
Int Immunopharmacol Pentraxin 3 promotes microglial M2 polarization and excitatory synapse formation in the hippocampus in depression |
