Recombinant Human PTH1R protein (rFc Tag)
种属
Human
纯度
>90 %, SDS-PAGE
标签
rFc Tag
生物活性
未测试
验证数据展示
产品信息
| 纯度 | >90 %, SDS-PAGE |
| 内毒素 | <0.1 EU/μg protein, LAL method |
| 生物活性 |
Not tested |
| 来源 | HEK293-derived Human PTH1R protein Asp27-Gly188 (Accession# Q03431) with a rabbit IgG Fc tag at the C-terminus. |
| 基因ID | 5745 |
| 蛋白编号 | Q03431 |
| 预测分子量 | 44.2 kDa |
| SDS-PAGE | 47-65 kDa, reducing (R) conditions |
| 组分 | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| 复溶 | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| 储存条件 |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
背景信息
PTH1R (Parathyroid Hormone 1 Receptor) is a class B1 G protein-coupled receptor (GPCR) that serves as the principal mediator of calcium and phosphate homeostasis by responding to two endogenous peptide ligands—parathyroid hormone (PTH) and parathyroid hormone-related protein (PTHrP). Predominantly expressed in kidney, bone, and breast tissue, PTH1R activation triggers divergent intracellular signaling cascades, including the Gαs-adenylyl cyclase-cAMP-PKA pathway and the Gαq-PLC-PKC axis, thereby regulating renal calcium reabsorption, bone remodeling, and skeletal development. Germline mutations in PTH1R cause contrasting skeletal disorders: constitutively activating mutations lead to Jansen-type metaphyseal chondrodysplasia (hypercalcemia and short stature), while inactivating mutations result in Blomstrand lethal chondrodysplasia. In adult medicine, intermittent PTH1R activation by teriparatide or abaloparatide is exploited pharmacologically to promote bone formation in osteoporosis, underscoring the receptor's dual significance in both physiological mineral ion balance and clinical therapeutics.
参考文献:
1.Alekos, Nathalie S et al. JCI insight vol. 8,6 (2023): e165604. 2.Culhane, Kelly J et al. ACS chemical biology vol. 13,8 (2018): 2347-2358. 3.Shimomura-Kuroki, Junko et al. Odontology vol. 105,2 (2017): 150-154. 4.Kim, Beomchang et al. Experimental and therapeutic medicine vol. 22,6 (2021): 1379. 5.Nampoothiri, Sheela et al. The Journal of clinical endocrinology and metabolism vol. 101,11 (2016): 4283-4289.
