Human PRAME ELISA Kit
Sensitivity
0.69 ng/mL
Range
3.13-200 ng/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE01000 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human PRAME ELISA kit is to be used to detect and quantify protein levels of endogenous human PRAME. The assay recognizes human PRAME. An antibody specific for human PRAME has been pre-coated onto the microwells. The human PRAME protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human PRAME is added to detect the captured human PRAME protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human PRAME ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.69 ng/mL |
| Range | 3.13-200 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 23532 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 72% | 71%-74% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 94.99 | 7.51 | 7.91 |
| 2 | 8 | 22.47 | 1.67 | 7.44 |
| 3 | 8 | 6.25 | 0.50 | 7.97 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 89.91 | 9.66 | 10.74 |
| 2 | 16 | 21.87 | 1.81 | 8.27 |
| 3 | 16 | 6.21 | 0.50 | 8.08 |
Background Information
PRAME (PReferentially expressed Antigen in MElanoma) is a tumor-associated antigen that was first identified through analysis of the specificity of tumor-reactive T-cell clones derived from a patient with metastatic cutaneous melanoma. It was subsequently found that PRAME is not only expressed in cutaneous melanoma, but also ocular melanoma and various nonmelanocytic malignant neoplasms, including non-small cell lung cancer, breast carcinoma, renal cell carcinoma, ovarian carcinoma, leukemia, synovial sarcoma, and myxoid liposarcoma.Normal healthy tissues are not known to express PRAME except for testis, ovary, placenta, adrenals, and endometrium. The expression of PRAME is a marker for an undifferentiated cellular state and has been implicated in the inhibition of tumour cell differentiation and the promotion of proliferation, which can contribute to oncogenesis .
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | MAPE, OIP 4, OIP4, OIP-4, Opa-interacting protein 4 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
