Human PML ELISA Kit
Sensitivity
0.04 ng/mL
Range
0.156-10 ng/mL
Reactivity
Human
Cat no : KE00934
Synonyms
E3 SUMO-protein ligase PML, EC:2.3.2.-, Protein PML, RING-type E3 SUMO transferase PML, RNF71
Validation Data Gallery
Product Information
KE00934 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human PML ELISA kit is to be used to detect and quantify protein levels of endogenous human PML. The assay recognizes human PML. An antibody specific for human PML has been pre-coated onto the microwells. The human PML protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human PML is added to detect the captured human PML protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human PML ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.04 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5371 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 89% | 82%-94% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 4.27 | 0.17 | 3.96 |
| 2 | 8 | 1.28 | 0.06 | 4.69 |
| 3 | 8 | 0.69 | 0.04 | 5.50 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 4.23 | 0.18 | 4.28 |
| 2 | 16 | 1.16 | 0.14 | 11.79 |
| 3 | 16 | 0.61 | 0.09 | 14.74 |
Background Information
PML, also named as MYL, RNF71 and TRIM19, is a member of RBCC/TRIM family of proteins that possess a RING finger domain, B-box, and coiled-coil domain. It functions as tumor suppressor. PML is required for normal, caspase-dependent apoptosis in response to DNA damage, FAS, TNF, or interferons. It plays a role in transcription regulation, DNA damage response, DNA repair and chromatin organization. PML plays a role in processes regulated by retinoic acid, regulation of cell division, terminal differentiation of myeloid precursor cells and differentiation of neural progenitor cells. It regulates PTEN compartmentalization through the inhibition of USP7-mediated deubiquitinylation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | E3 SUMO-protein ligase PML, EC:2.3.2.-, Protein PML, RING-type E3 SUMO transferase PML, RNF71 |
