Human PLA2G1B ELISA Kit
Sensitivity
0.08 ng/mL
Range
0.094 - 96 ng/mL
Reactivity
Human
Cat no : KE00098
Synonyms
Group IB phospholipase A2, Phospholipase A2, PLA2, PLA2A, PLA2G1B, PPLA2
Validation Data Gallery
Product Information
KE00098 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Human PLA2G1B ELISA kit is to be used to detect and quantify protein levels of endogenous human PLA2G1B. The assay recognizes human PLA2G1B. An antibody specific for human PLA2G1B has been pre-coated onto the microwells. The human PLA2G1B protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human PLA2G1B is added to detect the captured human PLA2G1B protein. For signal development,horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent.Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human PLA2G1B ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell culture supernatants, Cell lysates 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.08 ng/mL |
| Range | 0.094 - 96 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5319 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatants | 105% | 77%-126% |
| Cell lysates | 100% | 72%-115% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 0.69 | 0.05 | 6.7 |
| 2 | 20 | 9.55 | 0.87 | 9.1 |
| 3 | 20 | 92.38 | 9.37 | 10.1 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 0.64 | 0.04 | 6.9 |
| 2 | 24 | 9.45 | 0.59 | 6.3 |
| 3 | 24 | 78.98 | 6.95 | 8.8 |
Background Information
Phospholipase A2 group IB, also know as PLA2G1B, is a secreted member of the phospholipase A2 (PLA2) class of enzymes, is produced by the pancreatic acinar cells. PLA2G1B is a calcium-dependent enzyme which can catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to release arachidonic acid (AA) and lysophospholipids. PLA2G1B is involved in several physiological processes including cell contraction, cell proliferation and pathological response.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Group IB phospholipase A2, Phospholipase A2, PLA2, PLA2A, PLA2G1B, PPLA2 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
