Human/Mouse/Rat PIP5K1A ELISA Kit
Sensitivity
0.008 ng/mL
Range
0.156-10 ng/mL
Reactivity
Human/Mouse/Rat
Validation Data Gallery
Product Information
KE00227 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The PIP5K1A ELISA kit is to be used to detect and quantify protein levels of endogenous PIP5K1A. The assay recognizes human/mouse/rat PIP5K1A. An antibody specific for PIP5K1A has been pre-coated onto the microwells. The PIP5K1A protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for PIP5K1A is added to detect the captured PIP5K1A protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Mouse/Rat PIP5K1A ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Human serum, Human plasma, Mouse serum, Rat serum and Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.008 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human/Mouse/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 8394 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 87% | 80%-98% |
| Mouse serum | 102% | 76%-120% |
| Rat serum | 86% | 78%-97% |
| Cell lysate | 86% | 75%-99% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 0.30 | 0.02 | 8.4 |
| 2 | 20 | 1.17 | 0.03 | 2.6 |
| 3 | 20 | 4.92 | 0.14 | 2.9 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 0.28 | 0.02 | 6.5 |
| 2 | 24 | 1.20 | 0.08 | 7.0 |
| 3 | 24 | 5.57 | 0.38 | 6.8 |
Background Information
Phosphatidylinositol 4-phosphate 5-kinases (PIP5Ks) play diverse roles in the cellular biology of many organisms, including signal transduction, secretion and vesicular trafficking, and regulation of cytoskeleton assembly.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | PIP5K1 alpha, PIP5K1A, PIP5KIalpha, PtdIns(4)P 5 kinase 1 alpha |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
