Human PGA5 ELISA Kit
Sensitivity
0.16 ng/mL
Range
0.5-32 ng/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00084 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The PGA5 ELISA kit is to be used to detect and quantify protein levels of endogenous PGA5. The assay recognizes human PGA5. An antibody specific for PGA5 has been pre-coated onto the microwells. The PGA5 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for PGA5 is added to detect the captured PGA5 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human PGA5 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.16 ng/mL |
| Range | 0.5-32 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5222 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 93% | 75%-115% |
| Cell culture supernatants | 104% | 78%-127% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 24.36 | 1.33 | 5.5 |
| 2 | 20 | 6.43 | 0.33 | 5.2 |
| 3 | 20 | 0.65 | 0.06 | 8.9 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 23.79 | 1.86 | 7.8 |
| 2 | 24 | 5.78 | 0.49 | 8.5 |
| 3 | 24 | 0.48 | 0.04 | 9.0 |
Background Information
Pepsinogen I belongs to the peptidase A1 family. The gene endoces a 388 amino acid protein with a 15 amino acid signal peptide and 46 amino acid propeptide. Pepsinogen I is secreted only by the chief cells of the corpus, and its serum level decreases with increasing grade of the corpus atrophy (loss of oxyntic glands). Low serum levels of pepsinogen I are indicative of chronic atrophy, a risk factor for gastric cancer.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Pepsin A, PGA3, PGA4, PGA5 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
