Human PCSK9/Proprotein Convertase 9 ELISA Kit
Validation Data Gallery
Product Information
KE00181 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The PCSK9 ELISA kit is to be used to detect and quantify protein levels of endogenous PCSK9. The assay recognizes human PCSK9. An antibody specific for PCSK9 has been pre-coated onto the microwells. The PCSK9 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for PCSK9 is added to detect the captured PCSK9 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human PCSK9/Proprotein Convertase 9 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Cell lysates 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 255738 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 93% | 82%-105% |
| Cell culture supernatants | 87% | 79%-104% |
| Cell lysates | 105% | 86%-115% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 4.66 | 0.15 | 3.1 |
| 2 | 20 | 1.26 | 0.02 | 1.9 |
| 3 | 20 | 0.31 | 0.01 | 3.4 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 4.91 | 0.23 | 4.6 |
| 2 | 24 | 1.26 | 0.05 | 3.8 |
| 3 | 24 | 0.27 | 0.02 | 6.7 |
Background Information
PCSK9, also named as NARC1 and PC9, belongs to the peptidase S8 family. PCSK9 is expressed and secreted by several tissues but principally by the liver, small intestines, and kidney(PMID 28587771). It may be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis. PCSK9 is a regulator of the LDL receptor and plasma cholesterol concentrations(PMID: 26293463).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | PCSK9, EC:3.4.21.-, FH3, HCHOLA3, LDLCQ1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Biomed Pharmacother Novel ASO therapeutic target designed against hyperlipidemia via PCSK9 knockdown | ||
Mol Ther Methods Clin Dev Design and systematic evaluation of novel chemical modification patterns enable the identification of a potent siRNA targeting PCSK9. |
