Human PARK7/DJ-1 ELISA Kit
Sensitivity
0.06 ng/mL
Range
0.39-25 ng/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00341 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human PARK7/DJ-1 ELISA kit is to be used to detect and quantify protein levels of endogenous human PARK7/DJ-1. The assay recognizes human PARK7/DJ-1. An antibody specific for human PARK7/DJ-1 has been pre-coated onto the microwells. The human PARK7/DJ-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human PARK7/DJ-1 is added to detect the captured human PARK7/DJ-1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human PARK7/DJ-1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum,Plasma,Cell lysates,Cerebrospinal fluid 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.06 ng/mL |
| Range | 0.39-25 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 11315 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 105% | 81%-124% |
| Cell lysate | 94% | 83%-104% |
| Human cerebrospinal fluid (CSF) | 97% | 83%-119% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 13.10 | 0.40 | 3.06 |
| 2 | 20 | 2.94 | 0.07 | 2.24 |
| 3 | 20 | 0.76 | 0.04 | 4.85 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 13.25 | 1.04 | 7.88 |
| 2 | 24 | 2.84 | 0.19 | 6.54 |
| 3 | 24 | 0.75 | 0.07 | 9.68 |
Background Information
PARK7, also named as DJ1, belongs to the peptidase C56 family. It protects cells against oxidative stress and cell death. PARK7 plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. It eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. PARK7 has cell-growth promoting activity and transforming activity. It may function as a redox-sensitive chaperone. It's precursor undergoes a cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | PARK7, PARK7,DJ-1, DJ 1, DJ1, DJ-1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
