Human P21 ELISA Kit
Cat no : KE00049
Synonyms
CAP20, CDK interacting protein 1, CDKN1, CDKN1A, CIP1, MDA 6, MDA6, P21, P21;CDKN1A, p21CIP1, SDI1, WAF1
Validation Data Gallery
Product Information
KE00049 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The P21 ELISA kit is designed for the quantitative determination of P21 in samples of human origin. An antibody specific for P21 has been pre-coated onto the microwells. The P21 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for P21 is added to detect the captured P21 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human P21 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysates 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.1 ng/mL |
| Range | 0.25-16 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1026 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysates | 87% | 81%-97% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 10.29 | 0.201 | 2.0 |
| 2 | 20 | 3.05 | 0.070 | 2.3 |
| 3 | 20 | 0.63 | 0.025 | 4.0 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 10.24 | 0.346 | 3.4 |
| 2 | 24 | 3.08 | 0.094 | 3.1 |
| 3 | 24 | 0.67 | 0.052 | 7.8 |
Background Information
P21 (also known as CIP1, WAF1) is a cyclin-dependent kinase inhibitor. P21 binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of P21 is induced by wild-type but not mutant p53 protein, through which P21 mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. P21 can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. P21 was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CAP20, CDK interacting protein 1, CDKN1, CDKN1A, CIP1, MDA 6, MDA6, P21, P21;CDKN1A, p21CIP1, SDI1, WAF1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Intervirology New potential miRNA biomarkers in HIV Elite Controllers, HIV infections and Co-Infections with HBV or HCV | ||
Biomolecules Correlation of Occupational Exposure to Carcinogenic Polycyclic Aromatic Hydrocarbons (cPAHs) and Blood Levels of p53 and p21 Proteins. | ||
Analyst Sensitive and selective monitoring of the DNA damage-induced intracellular p21 protein and unraveling the role of the p21 protein in DNA repair and cell apoptosis by surface plasmon resonance. | ||
Cureus The Association of Increased Oxidative Stress and Tumor Biomarkers Related to Polyaromatic Hydrocarbons Exposure for Different Occupational Workers in Makkah, Saudi Arabia |
