Human P16-INK4A ELISA Kit
Sensitivity
0.45 ng/mL
Range
1.56-100 ng/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00706 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human P16-INK4A ELISA kit is to be used to detect and quantify protein levels of endogenous human P16-INK4A. The assay recognizes human P16-INK4A. An antibody specific for human P16-INK4A has been pre-coated onto the microwells. The human P16-INK4A protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human P16-INK4A is added to detect the captured human P16-INK4A protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human P16-INK4A ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.45 ng/mL |
| Range | 1.56-100 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1029 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 91% | 82%-103% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 44.94 | 3.43 | 7.64 |
| 2 | 8 | 9.24 | 0.67 | 7.26 |
| 3 | 8 | 5.05 | 0.37 | 7.24 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 47.56 | 4.68 | 9.84 |
| 2 | 16 | 9.94 | 1.12 | 11.29 |
| 3 | 16 | 5.52 | 0.57 | 10.28 |
Background Information
CDKN2A encodes two transcripts with alternative transcriptional start sites. Both transcripts share exons 2 and 3, but are translated in different open reading frames to yield two distinct proteins: p16INK4a and ARF (p14ARF in humans, p19ARF in mice). The tumor suppressor protein p16Ink4a (encoded from the CDKN2A locus) is often transcriptionally activated in cells undergoing senescence and is one of the main regulators of this program, and it is upregulated in multiple tissues during aging. p16-Ink4a is the principal member of the Ink4 family of CDK inhibitors. p16-Ink4a contributes to the regulation of cell cycle progression by inhibiting the S phase. p16Ink4a binds to CDK4/6, inhibiting cyclin D-CDK4/6 complex formation and CDK4/6-mediated phosphorylation of Rb family members. Expression of p16-Ink4a maintains the Rb family members in a hypophosphorylated state, which promotes binding to E2F1 and leads to G1 cell cycle arrest.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CDKN2A/P16-INK4A, P16 INK4A, CDKN2A, MTS1, CDK4I |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
